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Species: Saccharomyces cerevisiae
Genetic Insert: pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15329733
Comments: DNAs for Vps25, Vps22, and Vps36 were cloned in order into pST39. Vps22 was tagged with an N-terminal hexahistidine tag and a TEV protease cleavage site; the other subunits were untagged.
Proper citation: RRID:Addgene_17633 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Bro1 (1-387)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15935782
Comments: The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17635 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ub-MPGLW-Fbp1
Vector Backbone Description: Backbone Marker:Alexander Varshavsky's lab; Backbone Size:8017; Vector Backbone:pJO629; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513738
Proper citation: RRID:Addgene_176622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: bar1Sc
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176738 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mfalpha1Sc
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176720 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-FUS1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176706 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-DIG2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176705 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-FUS3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176707 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABP1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gal4 activation domain plus GGSGGGGSGG linker
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard, derived from GB0900
Proper citation: RRID:Addgene_177034 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RBD2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AUR1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ENT5
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STV1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177110 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+5
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17718 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pRS306Sup35R1
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17715 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+2
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+4
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17717 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+7
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17720 Copy
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