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Species: Mycobcterium
Genetic Insert: Integrase from pMV361
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11310445
Proper citation: RRID:Addgene_50000 Copy
Vector Backbone Description: Backbone Marker:Messing Lab; Backbone Size:2680; Vector Backbone:pUC6, Addgene plasmid #49793; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6323249
Proper citation: RRID:Addgene_50002 Copy
Vector Backbone Description: Backbone Marker:Messing Lab; Backbone Size:2686; Vector Backbone:pUC6, Addgene plasmid #49793; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6323249
Comments: Identical to pUC18 (Addgene plasmid #50004) except that the multiple cloning sites (MCS) are arranged in opposite orientation.
Proper citation: RRID:Addgene_50005 Copy
Species: Homo sapiens
Genetic Insert: ring finger protein 31
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5191; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060851
Proper citation: RRID:Addgene_50015 Copy
Species: Homo sapiens
Genetic Insert: SHANK-associated RH domain interactor
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5171; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060851
Proper citation: RRID:Addgene_50014 Copy
Species: Synthetic
Genetic Insert: G-CaMP3
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23364746
Comments: NOTE: There are some discrepancies between the Addgene quality control sequence and the depositor's full assembled sequence. These differences are not in functionally relevant parts of the plasmid.
Proper citation: RRID:Addgene_50022 Copy
Species: Synthetic
Genetic Insert: Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
Vector Backbone Description: Backbone Size:4086; Vector Backbone:pCS2+; Vector Types:Multi-purpose expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_50020 Copy
Species: Streptococcus pyogenes
Genetic Insert: SrtA(strep)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22348280
Proper citation: RRID:Addgene_50033 Copy
Species: Streptococcus pyogenes
Genetic Insert: HA-SrtA(strep)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22348280
Proper citation: RRID:Addgene_50032 Copy
Species: HPV
Genetic Insert: HPV 16 E6no*
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pNCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Comments: *The V42L mutation prevents the internal splicing of the DNA so only the "no *" (unspliced form) of 18E6 is produced.
Proper citation: RRID:Addgene_47706 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47709 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Comments: Gene was obtained from collaborator Andor Kiss, Miami University of Ohio.
Proper citation: RRID:Addgene_47666 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47664 Copy
Species: Homo sapiens
Genetic Insert: Olfactomedin-like domain with V426F mutation
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21612213
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47702 Copy
Species: Homo sapiens
Genetic Insert: Olfactomedin-like domain with I499F mutation
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21612213
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47703 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47667 Copy
Genetic Insert: PesaR-C
Vector Backbone Description: Backbone Size:8891; Vector Backbone:pET-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-C (274 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pET-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pET-PesaRlux for more details and plasmid map.
Proper citation: RRID:Addgene_47658 Copy
Genetic Insert: PesaR-D
Vector Backbone Description: Backbone Size:8891; Vector Backbone:pET-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-D (275 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pET-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pET-PesaRlux for more details and plasmid map.
Proper citation: RRID:Addgene_47657 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4580; Vector Backbone:pKK233-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1334432
Proper citation: RRID:Addgene_47599 Copy
Species: C. reinhardtii
Genetic Insert: hChR2 (H134R)
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47633 Copy
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