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Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Comments: HCT116 has a 3-bp deletion that removes one amino acid (Ser45).
Proper citation: RRID:Addgene_16520 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10555149
Proper citation: RRID:Addgene_16539 Copy
Genetic Insert: 4xACO
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10555149
Comments: Contains 4 copies of the prototypic PPAR-responsive element ACO (5'-AGGACAAAGGTCA-3'). The prototypic PPAR response element ACO from the acyl-CoA oxidase gene promotor contains two copies of the core binding sequence AGGTCA separated by one base pair.
Proper citation: RRID:Addgene_16533 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
Species: Homo sapiens
Genetic Insert: p53 binding site
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4900; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659898
Comments: This reporter contains 3 copies of the wt p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGCAT TAGCCCAGAC ATGTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA CATGTCTGGG CTAATGCTAC AGGGTAC-3' for BDS-2.
Proper citation: RRID:Addgene_16515 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
Proper citation: RRID:Addgene_16514 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pcDNA?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
Proper citation: RRID:Addgene_16512 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Comments: SW48 has a C-to-A missense mutation that changes Ser33 to Tyr.
Note that the NheI site is not intact and cannot be used for sub-cloning.
Proper citation: RRID:Addgene_16519 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: COV E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165123 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF3a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165121 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF9b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165122 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165120 Copy
Vector Backbone Description: Backbone Size:2790; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD129.36, Ligation number L4440. "Author's Map" courtesy of Dr. Guy Caldwell.
Proper citation: RRID:Addgene_1654 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165112 Copy
Species: Homo sapiens
Genetic Insert: FCER1G
Vector Backbone Description: Backbone Marker:Mark M Davis; Backbone Size:6600; Vector Backbone:pBJ1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2138619
Comments: This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert.
Proper citation: RRID:Addgene_16540 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165134 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Orf3a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165138 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp13
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165136 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp14
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165137 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 spike protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165096 Copy
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