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Species: Saccharomyces cerevisiae
Genetic Insert: CFP-FKBP-Inp54p
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16990515
Proper citation: RRID:Addgene_20155 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UPR-RFP
Vector Backbone Description: Backbone Size:4835; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19026441
Comments: There is a 2 base gap between depositor's sequence and Addgene sequence; per depositor this gap is in the promoter region and shouldn't significantly affect the plasmid.
Proper citation: RRID:Addgene_20132 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eroGFP
Vector Backbone Description: Backbone Size:4835; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19026441
Proper citation: RRID:Addgene_20131 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO-conjugating enzyme Ubc9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_20369 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLPe recombinase
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pBS; Vector Types:Mammalian Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16400644
Comments: Please note that this plasmid runs as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you need the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_20733 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_21052 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_21051 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pCY3010-02; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22473760
Proper citation: RRID:Addgene_36211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DMC1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-11a/pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11551925
Comments: See the attached maps for complete cloning details.
Proper citation: RRID:Addgene_36451 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD54
Vector Backbone Description: Backbone Marker:K Runge; Backbone Size:7011; Vector Backbone:YepFAT4; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10526232
Proper citation: RRID:Addgene_36452 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScLEU2
Vector Backbone Description: Vector Backbone:L22-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36910 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScURA3
Vector Backbone Description: Backbone Size:9951; Vector Backbone:pSinRep5; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36913 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScURA3
Vector Backbone Description: Backbone Size:9987; Vector Backbone:L22-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36909 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COF1-RFP
Vector Backbone Description: Vector Backbone:pRS415-LEU,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332110
Proper citation: RRID:Addgene_37102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DAD4
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Comments: The sequence for DAD4 mRNA was taken from yeast.utgenome.org. Two G bases were added at the 5´-ends of the mRNA in order to promote efficient transcription and the sequence GGATC was added at the 3´-end as part of a BamHI site during cloning.
Proper citation: RRID:Addgene_37224 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF4A
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37225 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF3
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF3
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL41A
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Comments: The sequence of RPL41A mRNA is as previously described (Yu and Warner, 2001). Two G bases were added at the 5´-ends of both mRNAs in order to promote efficient transcription and the sequence GGATC was added at the 3´-end as part of a BamHI site during cloning.
Proper citation: RRID:Addgene_37223 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF1A
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637
Proper citation: RRID:Addgene_37218 Copy
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