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Species: Synthetic
Genetic Insert: Lyn Fak biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21792185
Comments: To observe the FAK activity at DRM regions, Lyn–FAK biosensor was constructed by fusing a DRM-targeting motif containing myristoylation and palmitoylation sites (glycine and cysteine) derived from Lyn, at the N-terminus of the cytosolic FAK (Cyto-FAK) biosensor (Addgene plasmid 78300).
Proper citation: RRID:Addgene_78299 Copy
Species: Synthetic
Genetic Insert: Murine leukemia reverse transcriptase
Vector Backbone Description: Backbone Size:3514; Vector Backbone:pVRb; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27091073
Proper citation: RRID:Addgene_78234 Copy
Species: Synthetic
Genetic Insert: mutated miR-34 target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26996824
Proper citation: RRID:Addgene_78259 Copy
Species: Synthetic
Genetic Insert: fully complimentary miR-34 target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26996824
Comments: Please note there is a single nucleotide mismatch between the published miR34 sequence and Addgene's quality control sequence. This mismatch does not affect plasmid function.
Proper citation: RRID:Addgene_78258 Copy
Species: Synthetic
Genetic Insert: TurboFP635
Vector Backbone Description: Backbone Marker:Seeliger et al., 2012 PLoS One. 7(1):e29266. Epub 2012 Jan 18; Vector Backbone:pST5552; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacterial replicating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27027532
Proper citation: RRID:Addgene_78314 Copy
Species: Synthetic
Genetic Insert: mutated let-7 target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26996824
Proper citation: RRID:Addgene_78261 Copy
Species: Synthetic
Genetic Insert: FAT FAK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24222685
Proper citation: RRID:Addgene_78303 Copy
Species: Synthetic
Genetic Insert: KRas FAK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21792185
Comments: A polybasic-geranylgeranyl motif (cysteine) adapted from K-Ras, was fused at the carboxy terminus of the Cyto-FAK biosensor (Addgene plasmid 78300) for the generation of KRas–FAK biosensor that can localize at the general membrane area outside DRM regions
Proper citation: RRID:Addgene_78301 Copy
Species: Synthetic
Genetic Insert: F3-flanked spectinomycin resistance cassette
Vector Backbone Description: Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38488830
Comments: Please visit https://doi.org/10.1101/2024.01.17.576040 for bioRxiv preprint.
Proper citation: RRID:Addgene_127551 Copy
Species: Synthetic
Genetic Insert: F5-flanked tetracycline resistance cassette
Vector Backbone Description: Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127552 Copy
Species: Synthetic
Genetic Insert: PtetO7.1_Flagtag_8Glycine linker
Vector Backbone Description: Backbone Size:2894; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127581 Copy
Species: Synthetic
Genetic Insert: PtetO7.1_Flagtag_8Glycine linker
Vector Backbone Description: Backbone Size:3350; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127580 Copy
Species: Synthetic
Genetic Insert: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Vector Backbone Description: Backbone Size:3748; Vector Backbone:pRG203MX; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127577 Copy
Species: Synthetic
Genetic Insert: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Vector Backbone Description: Backbone Size:5108; Vector Backbone:pRG206; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127575 Copy
Species: Synthetic
Genetic Insert: 5HT2A_NB2
Vector Backbone Description: Vector Backbone:pCDNA3.1 hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127690 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-R72K
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127683 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y66F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127681 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y63F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127680 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y98F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127686 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y76F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127684 Copy
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