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Species: Other
Genetic Insert: AKAR5Δ
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30100256
Proper citation: RRID:Addgene_119912 Copy
Species: Other
Genetic Insert: tAKAR4α
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30100256
Proper citation: RRID:Addgene_119919 Copy
Species: Other
Genetic Insert: bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
Vector Backbone Description: Vector Backbone:pCAP03; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120232 Copy
Species: Other
Genetic Insert: promoter cassette containing act1p promoter and apramycin resistance gene
Vector Backbone Description: Vector Backbone:pCAP03; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120233 Copy
Species: Other
Genetic Insert: promoter cassette containing ermE*p promoter and ampicillin resistance gene
Vector Backbone Description: Vector Backbone:pCAP03; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120234 Copy
Species: Other
Genetic Insert: mClover3/mScarletP2A-mcs
Vector Backbone Description: Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mClover3 can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
The associated publication utilized mNeon instead of sfGFP/mClover3, but mNeon has is exceedingly difficult to share. Therefore, these two plasmids (120261 and 120262) are made available, using sfGFP and mClover3 instead of mNeon. These plasmids have been tested, and are equivalent to the mNeon variant.
Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120262 Copy
Species: Other
Genetic Insert: mNeptune2
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120337 Copy
Species: Other
Genetic Insert: Rosa26 gRNA (SpCas9 scaffold)
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120296 Copy
Species: Other
Genetic Insert: mWasabi
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120331 Copy
Species: Other
Genetic Insert: AcrIIA4-2xmiR-1 binding sites
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120299 Copy
Species: Other
Genetic Insert: mCardinal
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120333 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120326 Copy
Species: Other
Genetic Insert: mClover
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Comments: D103N in mClover does not affect plasmid function
Proper citation: RRID:Addgene_120328 Copy
Species: Other
Genetic Insert: Lux operon
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120323 Copy
Species: Other
Genetic Insert: Lux operon
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120324 Copy
Species: Other
Genetic Insert: Ilmol
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30315245
Proper citation: RRID:Addgene_120311 Copy
Species: Other
Genetic Insert: AcrIIC1
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120300 Copy
Species: Other
Genetic Insert: AcrIIC3-2xmiR-122 binding sites
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120303 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25636838
Comments: an arabinose-inducible promoter to express Cas9
Proper citation: RRID:Addgene_120423 Copy
Species: Other
Genetic Insert: T3_Tef
Vector Backbone Description: Vector Backbone:pCR4Blunt-TOPO; Vector Types:Cloning/assembly plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31148366
Proper citation: RRID:Addgene_120776 Copy
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