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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET His6 Mocr TEV expression vector with BioBrick polypromoter restriction sites (14-O) Resource Report Resource Website |
RRID:Addgene_48310 | Ampicillin | This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV cleavable His6-Mocr tag on the N-terminus and Amp resistance. To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase for LIC, use dCTP for insert and dGTP for vector. The Series-14 plasmids were designed to overcome the unpredictable expression patterns of genes from a polycistronic message. As mentioned in the Series-2 and Series-9 polycistronic sections, often genes that express well from a single gene mRNA do not express at the same level when placed in a polycistronic message next to other genes. This effect is likely due to mRNA structure interfering with access to the ribosome binding site. When we first made our polypromoter plasmids we found that we could clone multiple genes together in the Xl1-Blue cells so that each possess a T7 promoter, followed by a Lac operator, followed by a ribosome binding site, followed by your open reading frame (T7-LacO-RBS-yORF). However, these multi-gene polypromoter plasmids were unable to be transformed into an expression strain such as BL-21 or Rosetta2. We then figured out that we needed a RecAexpression strain (like Rosetta-Blues) to successfully propagate our polypromoter plasmids. Although Rosetta-Blue cells propagated our plasmids, expression was not so great and we didn't want to rely on a specialized strain to perform our expression. Finally, we figured out that it was the LacO that follows the T7 promoter that was causing plasmid instability in our polypromoter system. Removal of the LacO allowed us to express our polypromoter plasmids in Rosetta2 cells and we have had much success with these plasmids since. Much like our Series- 2 plasmids, the Series-14 plasmids are going to give a substantial amount of leaky expression. However, even with this shortcoming, we have found Series-14 to be a valuable resource for expressing multi-protein complexes. Cloning into the Series-14 plasmids and subsequent biobrick subcloning is performed exactly as described for the Series-9 plasmids. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:3430; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | ||||
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pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q) Resource Report Resource Website 1+ mentions |
RRID:Addgene_48311 | Ampicillin | This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV cleavable MBP tag on the N-terminus and Amp resistance. To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase for LIC, use dCTP for insert and dGTP for vector. The Series-14 plasmids were designed to overcome the unpredictable expression patterns of genes from a polycistronic message. As mentioned in the Series-2 and Series-9 polycistronic sections, often genes that express well from a single gene mRNA do not express at the same level when placed in a polycistronic message next to other genes. This effect is likely due to mRNA structure interfering with access to the ribosome binding site. When we first made our polypromoter plasmids we found that we could clone multiple genes together in the Xl1-Blue cells so that each possess a T7 promoter, followed by a Lac operator, followed by a ribosome binding site, followed by your open reading frame (T7-LacO-RBS-yORF). However, these multi-gene polypromoter plasmids were unable to be transformed into an expression strain such as BL-21 or Rosetta2. We then figured out that we needed a RecAexpression strain (like Rosetta-Blues) to successfully propagate our polypromoter plasmids. Although Rosetta-Blue cells propagated our plasmids, expression was not so great and we didn't want to rely on a specialized strain to perform our expression. Finally, we figured out that it was the LacO that follows the T7 promoter that was causing plasmid instability in our polypromoter system. Removal of the LacO allowed us to express our polypromoter plasmids in Rosetta2 cells and we have had much success with these plasmids since. Much like our Series- 2 plasmids, the Series-14 plasmids are going to give a substantial amount of leaky expression. However, even with this shortcoming, we have found Series-14 to be a valuable resource for expressing multi-protein complexes. Cloning into the Series-14 plasmids and subsequent biobrick subcloning is performed exactly as described for the Series-9 plasmids. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:4151; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 1 | ||||
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Gal1/10 His6 TEV Ura S. cerevisiae expression vector (12URA-B) Resource Report Resource Website 1+ mentions |
RRID:Addgene_48304 | Ampicillin | Note: The full plasmid sequence displayed here is theoretical and may not be entirely accurate. Addgene's quality control sequencing has identified some discrepancies with the sequence, but they do not impact the plasmid's function. This plasmid is a LIC-adapted S. cerevisiae vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once. This vector has a TEV cleavable His6 tag on the N-terminus and can complement Ura auxotrophy. Add the following tags to your PCR primers: LicV1 Forward Tag TACTTCCAATCCAATGCA(ATG) LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP.Series 12 vectors are galactose inducible (using the Gal 1/10 promoter). The plasmids are cloned and propagated in E. coli. Plasmids are available to complement the Ade, Trp, or Ura auxotrophies. The Ade, Trp, and Ura plasmids can co-exist in the same yeast cell with no compatibility issues. We have successfully expressed 3 proteins from these 3 plasmids in the same strain (BCY123: Ade-, Trp-, Ura-).For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:8937; Vector Backbone:pRS426; Vector Types:NA; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 4 | ||||
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Gal1/10 His6-MBP TEV Trp S. cerevisiae expression vector (12TRP-C) Resource Report Resource Website |
RRID:Addgene_48302 | Ampicillin | Note: The full plasmid sequence displayed here is theoretical and may not be entirely accurate. Addgene's quality control sequencing has identified some discrepancies with the sequence, but they do not impact the plasmid's function. This plasmid is a LIC-adapted S. cerevisiae vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once. This vector has a TEV cleavable His6-MBP tag on the N-terminus and can complement Trp auxotrophy. Add the following tags to your PCR primers: LicV1 Forward Tag TACTTCCAATCCAATGCA(ATG) LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP. Series 12 vectors are galactose inducible (using the Gal 1/10 promoter). The plasmids are cloned and propagated in E. coli. Plasmids are available to complement the Ade, Trp, or Ura auxotrophies. The Ade, Trp, and Ura plasmids can co-exist in the same yeast cell with no compatibility issues. We have successfully expressed 3 proteins from these 3 plasmids in the same strain (BCY123: Ade-, Trp-, Ura-). For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:7773; Vector Backbone:pRS424; Vector Types:NA; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | ||||
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Gal1/10 His6-MBP TEV Ura S. cerevisiae expression vector (12URA-C) Resource Report Resource Website 1+ mentions |
RRID:Addgene_48305 | Ampicillin | Note: The full plasmid sequence displayed here is theoretical and may not be entirely accurate. Addgene's quality control sequencing has identified some discrepancies with the sequence, but they do not impact the plasmid's function. This plasmid is a LIC-adapted S. cerevisiae vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once. This vector has a TEV cleavable His6-MBP tag on the N-terminus and can complement Ura auxotrophy. Add the following tags to your PCR primers: LicV1 Forward Tag TACTTCCAATCCAATGCA(ATG) LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP. Series 12 vectors are galactose inducible (using the Gal 1/10 promoter). The plasmids are cloned and propagated in E. coli. Plasmids are available to complement the Ade, Trp, or Ura auxotrophies. The Ade, Trp, and Ura plasmids can co-exist in the same yeast cell with no compatibility issues. We have successfully expressed 3 proteins from these 3 plasmids in the same strain (BCY123: Ade-, Trp-, Ura-). For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:10092; Vector Backbone:pRS426; Vector Types:NA; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 2 | ||||
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Gal1/10 Ura S. cerevisiae expression vector (12URA-U) Resource Report Resource Website |
RRID:Addgene_48306 | Ampicillin | Note: The full plasmid sequence displayed here is theoretical and may not be entirely accurate. Addgene's quality control sequencing has identified some discrepancies with the sequence, but they do not impact the plasmid's function. This plasmid is a LIC-adapted S. cerevisiae vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once. This vector can complement Ura auxotrophy. Add the following tags to your PCR primers: Note: You must add an ATG to the beginning of your open reading frame. LICvBac Forward Tag TACTTCCAATCCAATCG LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP. Series 12 vectors are galactose inducible (using the Gal 1/10 promoter). The plasmids are cloned and propagated in E. coli. Plasmids are available to complement the Ade, Trp, or Ura auxotrophies. The Ade, Trp, and Ura plasmids can co-exist in the same yeast cell with no compatibility issues. We have successfully expressed 3 proteins from these 3 plasmids in the same strain (BCY123: Ade-, Trp-, Ura-). For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:8878; Vector Backbone:pRS426; Vector Types:NA; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | ||||
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pJH104 Resource Report Resource Website |
RRID:Addgene_48262 | TEF1p 5' fragment | Saccharomyces cerevisiae | Ampicillin | PMID:24604451 | Created by 4-part ligation with XhoI/EagI cut pBluescript II SK (-). Inserts were XhoI/XbaI TEF1p, XbaI/BamHI URA3, and BamHI/EagI TEF1p. | Backbone Marker:Agilent Technologies; Backbone Size:2961; Vector Backbone:pBluescript II SK (-); Vector Types:Routine cloning vector; Bacterial Resistance:Ampicillin | From SK1 background. -460 to -128 relative to TEF1 ORF | 2026-08-15 01:15:48 | 0 |
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Gal1/10 Ade S. cerevisiae expression vector (12ADE-U) Resource Report Resource Website |
RRID:Addgene_48300 | Ampicillin | Note: The full plasmid sequence displayed here is theoretical and may not be entirely accurate. Addgene's quality control sequencing has identified some discrepancies with the sequence, but they do not impact the plasmid's function. This plasmid is a LIC-adapted S. cerevisiae vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once. This vector can complement Ade auxotrophy. Add the following tags to your PCR primers: Note: You must add an ATG to the beginning of your open reading frame. LICvBac Forward Tag TACTTCCAATCCAATCG LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP. Series 12 vectors are galactose inducible (using the Gal 1/10 promoter). The plasmids are cloned and propagated in E. coli. Plasmids are available to complement the Ade, Trp, or Ura auxotrophies. The Ade, Trp, and Ura plasmids can co-exist in the same yeast cell with no compatibility issues. We have successfully expressed 3 proteins from these 3 plasmids in the same strain (BCY123: Ade-, Trp-, Ura-). For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:8429; Vector Backbone:pRS422; Vector Types:NA; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | ||||
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pJH124 Resource Report Resource Website |
RRID:Addgene_48259 | URA3 3' fragment | Saccharomyces cerevisiae | Ampicillin | PMID:24604451 | pJH124 was created by cloning an XbaI/XhoI Ag TEF1 promoter fragment into the same sites of vector IpO. The cloned insert was confirmed by DNA sequencing. | Backbone Marker:Agilent Technologies; Backbone Size:2961; Vector Backbone:pBluescript II SK (-); Vector Types:Routine cloning vector; Bacterial Resistance:Ampicillin | URA3 ORF from +216 to 80 bp downstream of the stop codon | 2026-08-15 01:15:49 | 0 |
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pAC154-dual-dCas9VP160-sgExpression Resource Report Resource Website 10+ mentions |
RRID:Addgene_48240 | dCas9 | Synthetic | Ampicillin | PMID:23979020 | Clone sgRNA spacer into BbsI site. Sequence using LKO5' primer: GACTATCATATGCTTACCGT For more information including protocols and updates, please go to http://www.crispr-on.org | Vector Backbone:pX335 (Addgene #42335); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | D10A H840A | 2026-08-15 01:15:48 | 24 |
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pHJ42 Resource Report Resource Website |
RRID:Addgene_48359 | Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR | T. brucei | Ampicillin | PMID:23954366 | For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html There are several mismatches between Addgene's quality control sequence and the reference sequence from the depositing lab; most notably in the beta-tubulin ORF, HSV thymidine kinase and the T. brucei Aldolase 3'UTR. These mismatches should not affect plasmid function. | Backbone Marker:Cross Lab; Vector Backbone:pHD309; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | |
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pDS69 Resource Report Resource Website |
RRID:Addgene_48367 | FLAG-intergenic region of TUB-HYG (modified from PMID 16269191) | T. brucei | Ampicillin | PMID:23954366 | For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html There are several mismatches between Addgene's quality control sequence and the reference sequence from the depositing lab. These mismatches should not affect plasmid function. | Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | |
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pHJ32 Resource Report Resource Website |
RRID:Addgene_48361 | Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR | T. brucei | Ampicillin | PMID:23954366 | For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html There are several mismatches between Addgene's quality control sequence and the reference sequence from the depositing lab; most notably in the beta-tubulin ORF, HSV thymidine kinase and the T. brucei Aldolase 3'UTR. These mismatches should not affect plasmid function. | Backbone Marker:Cross Lab; Vector Backbone:pHJ17; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | |
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pDS67 Resource Report Resource Website |
RRID:Addgene_48365 | 3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191) | T. brucei | Ampicillin | PMID:23954366 | For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html | Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:49 | 0 | |
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pCool-hDDB1-BPB Resource Report Resource Website |
RRID:Addgene_48639 | hDDB1-BPB | Homo sapiens | Ampicillin | PMID:16413485 | BL21 is recommended for protein overexpression. | Backbone Marker:pGex2T; Vector Backbone:pCool; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:52 | 0 | |
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roGFP_iR_pQE30 Resource Report Resource Website |
RRID:Addgene_48634 | roGFP1 | jellyfish | Ampicillin | PMID:23589496 | This plasmids needs to be amplified and maintained in M15 at all times as this strain has the pREP4 repressor vector that prevents undesired expression (it's important to maintain the plasmid when the protein is not expressed even if it's believed to be inert for the bacteria, to avoid selective pressure in favour of recombinant versions of the gene). | Backbone Size:3418; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | insertion R147B | 2026-08-15 01:15:53 | 0 |
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roGFP_iE_C147S_pQE30 Resource Report Resource Website |
RRID:Addgene_48631 | roGFP1 | jellyfish | Ampicillin | PMID:23589496 | This plasmids needs to be amplified and maintained in M15 at all times as this strain has the pREP4 repressor vector that prevents undesired expression (it's important to maintain the plasmid when the protein is not expressed even if it's believed to be inert for the bacteria, to avoid selective pressure in favour of recombinant versions of the gene). | Backbone Size:3418; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | insertion E147B, C147S | 2026-08-15 01:15:53 | 0 |
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pAcGHLT-B-DDB1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_48638 | DDB1 | Homo sapiens | Ampicillin | PMID:16413485 | Backbone Marker:Pharmagen; Vector Backbone:pAcGHLT-B; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:52 | 2 | ||
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pCS2TAL3-DDD Resource Report Resource Website 1+ mentions |
RRID:Addgene_48637 | Ampicillin | PMID:23667577 | Backbone Size:5768; Vector Backbone:pCS2; Vector Types:TALEN; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:53 | 2 | ||||
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pCS2TAL3-RRR Resource Report Resource Website 1+ mentions |
RRID:Addgene_48636 | Ampicillin | PMID:23667577 | Backbone Size:5798; Vector Backbone:pCS2; Vector Types:TALEN; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:52 | 2 |
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