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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Lyn FAK biosensor
 
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RRID:Addgene_78299 Lyn Fak biosensor Synthetic Ampicillin PMID:21792185 To observe the FAK activity at DRM regions, Lyn–FAK biosensor was constructed by fusing a DRM-targeting motif containing myristoylation and palmitoylation sites (glycine and cysteine) derived from Lyn, at the N-terminus of the cytosolic FAK (Cyto-FAK) biosensor (Addgene plasmid 78300). Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:20:23 3
pMLRT
 
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RRID:Addgene_78234 Murine leukemia reverse transcriptase Synthetic Kanamycin PMID:27091073 Backbone Size:3514; Vector Backbone:pVRb; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:20:22 0
psiCHECK2-miR-34 MT
 
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RRID:Addgene_78259 mutated miR-34 target site Synthetic Ampicillin PMID:26996824 Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin nucleotides 2-5 and 10-11 changed from CCGT to GGCA and GA to CT, respectively 2026-08-15 01:20:22 1
psiCHECK2-miR-34 WT
 
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RRID:Addgene_78258 fully complimentary miR-34 target site Synthetic Ampicillin PMID:26996824 Please note there is a single nucleotide mismatch between the published miR34 sequence and Addgene's quality control sequence. This mismatch does not affect plasmid function. Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin 2026-08-15 01:20:22 5
pTiGc
 
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RRID:Addgene_78314 TurboFP635 Synthetic Kanamycin PMID:27027532 Backbone Marker:Seeliger et al., 2012 PLoS One. 7(1):e29266. Epub 2012 Jan 18; Vector Backbone:pST5552; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacterial replicating vector; Bacterial Resistance:Kanamycin 2026-08-15 01:20:23 1
psiCHECK2-let-7 MT
 
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RRID:Addgene_78261 mutated let-7 target site Synthetic Ampicillin PMID:26996824 Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin nucleotides 2-5 and 10-11 changed from CTCC to GAGG and TC to AG, respectively 2026-08-15 01:20:22 1
FAT FAK biosensor
 
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RRID:Addgene_78303 FAT FAK biosensor Synthetic Ampicillin PMID:24222685 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:20:23 2
KRas FAK biosensor
 
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RRID:Addgene_78301 KRas FAK biosensor Synthetic Ampicillin PMID:21792185 A polybasic-geranylgeranyl motif (cysteine) adapted from K-Ras, was fused at the carboxy terminus of the Cyto-FAK biosensor (Addgene plasmid 78300) for the generation of KRas–FAK biosensor that can localize at the general membrane area outside DRM regions Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:20:23 0
pES134
 
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RRID:Addgene_127551 F3-flanked spectinomycin resistance cassette Synthetic Ampicillin PMID:38488830 Please visit https://doi.org/10.1101/2024.01.17.576040 for bioRxiv preprint. Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 0
pES133
 
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RRID:Addgene_127552 F5-flanked tetracycline resistance cassette Synthetic Ampicillin Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Multiple SNPs in tetA (originally from pBR322) to suppress a number of restriction sites 2026-08-15 01:04:37 0
FRP2375
 
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RRID:Addgene_127581 PtetO7.1_Flagtag_8Glycine linker Synthetic Ampicillin PMID:34342575 For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647. Backbone Size:2894; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
FRP2350
 
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1+ mentions
RRID:Addgene_127580 PtetO7.1_Flagtag_8Glycine linker Synthetic Ampicillin PMID:34342575 For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647. Backbone Size:3350; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 2
FRP2371
 
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RRID:Addgene_127577 PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1 Synthetic Ampicillin PMID:34342575 For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647. Backbone Size:3748; Vector Backbone:pRG203MX; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 3
FRP2365
 
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RRID:Addgene_127575 PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1 Synthetic Ampicillin PMID:34342575 For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647. Backbone Size:5108; Vector Backbone:pRG206; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
pcDNA3.1 5HT2A_NB2_1
 
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RRID:Addgene_127690 5HT2A_NB2 Synthetic Ampicillin PMID:31280962 These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. Vector Backbone:pCDNA3.1 hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:38 0
OphA-delta C6-R72K
 
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RRID:Addgene_127683 OphA-delta C6-R72K Synthetic Kanamycin PMID:30151425 Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin delta C6-R72K 2026-08-15 01:04:38 0
OphA-delta C6-Y66F
 
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RRID:Addgene_127681 OphA-delta C6-Y66F Synthetic Kanamycin PMID:30151425 Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin delta C6-Y66F 2026-08-15 01:04:38 0
OphA-delta C6-Y63F
 
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RRID:Addgene_127680 OphA-delta C6-Y63F Synthetic Kanamycin PMID:30151425 Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin delta C6-Y63F 2026-08-15 01:04:38 0
OphA-delta C6-Y98F
 
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RRID:Addgene_127686 OphA-delta C6-Y98F Synthetic Kanamycin PMID:30151425 Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin delta C6-Y98F 2026-08-15 01:04:39 0
OphA-delta C6-Y76F
 
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RRID:Addgene_127684 OphA-delta C6-Y76F Synthetic Kanamycin PMID:30151425 Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin delta C6-Y76F 2026-08-15 01:04:38 0

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