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Species: Saccharomyces cerevisiae
Genetic Insert: β′-COPI-WD40
Vector Backbone Description: Backbone Size:5731; Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35136165
Proper citation: RRID:Addgene_180753 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-APEX2
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, TEM imaging; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
Vector Backbone Description: Backbone Size:5954; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21558172
Comments: There is a single nucleotide (A) deletion in the sequence. This deletion does not affect the function of the plasmid.
Proper citation: RRID:Addgene_29699 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7267; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_29739 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35 gene encoding translation release factor 3 from S. cerevisiae
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29733 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35 gene encoding translation release factor 3 from S. cerevisiae
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29732 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35 gene encoding translation release factor 3 from S. cerevisiae
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29730 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7267; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_29742 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7267; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_29741 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7267; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_29740 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19266305
Comments: driven by Xenopus laevis heat shock 70 (hsp 70) promoter
Proper citation: RRID:Addgene_30521 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase tdTomato
Vector Backbone Description: Backbone Marker:Genbank DQ836146; Backbone Size:3007; Vector Backbone:I-SceI-pBSII-SK+; Vector Types:Flp/FRT, heat shock inducible, I-SceI Megabase vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19266305
Comments: FLP driven by Xenopus laevis heat shock 70 (hsp 70) promoter with CMV driven tdTomato, both in I-SceI megabase vector
Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.
Proper citation: RRID:Addgene_30523 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ulp1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2894; Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19031436
Comments: Mossessova E, Lima CD. Mol Cell. 2000 May;5(5):865-76.
Note: pFGET19_Ulp1 (#64697) is a new version of pHYRS52(#31122) with kanamycin resistance.
Proper citation: RRID:Addgene_31122 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TdTomato, GAL4
Vector Backbone Description: Vector Backbone:pBlueScriptSK; Vector Types:Mammalian Expression, Xenopus laevis expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385606
Comments: Additional references:
-Koster et. al, Dev Biol. 2001, 233(2):329-46. doi.org/10.1006/dbio.2001.0242
-Bestman, J.E., and Cline, H.T. PNAS. 2008, 105: 20494-20499. doi.org/10.1073/pnas.0806296105
-Gabmrill et. al, J Neurophysiol. 2018, 119: 1947–1961. doi.org/10.1152/jn.00051.2018
Proper citation: RRID:Addgene_161712 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast genes
Vector Backbone Description: Vector Backbone:ampl43; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247197
Proper citation: RRID:Addgene_161829 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSH2
Vector Backbone Description: Vector Backbone:eGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18077555
Proper citation: RRID:Addgene_161987 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR)
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_164574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET3pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164710 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164708 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1pr - rtTA - CYC1t
Vector Backbone Description: Backbone Marker:Zhou et al, Nature, 2018 PMID: 29562237; Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164706 Copy
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