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Genetic Insert: Term(fdx)-Ptet-gusA-Term(slpA)
Vector Backbone Description: Backbone Size:6179; Vector Backbone:pMTL960; Vector Types:E. coli - C. difficile shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21659510
Proper citation: RRID:Addgene_106367 Copy
Vector Backbone Description: Backbone Size:4952; Vector Backbone:pGL3-U6-sgRNA-PGK-puromycin (Addgene: 51133); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28964699
Proper citation: RRID:Addgene_106404 Copy
Genetic Insert: Ptet-himar1-Ter(slpA)-transposon
Vector Backbone Description: Backbone Size:6191; Vector Backbone:pMTL960; Vector Types:E. coli - C. difficile shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25714712
Proper citation: RRID:Addgene_106377 Copy
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Replaced the puromycin resistance gene from pBABE-puro with GFP for cell sorting. HindIII-ClaI GFP ORF from phrGFP-c (Stratagene) was cloned into pBABE-puro. hrGFP =humanized Renilla green fluorescent protein.
Proper citation: RRID:Addgene_10668 Copy
Species: Homo sapiens
Genetic Insert: PTEN shRNA
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Comments: siRNA sequence is ACTTGAAGGCGTATACAGGA.
Proper citation: RRID:Addgene_10669 Copy
Species: Homo sapiens
Genetic Insert: p53 shRNA
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12887925
Comments: Also reference: Stewart, S.A., Dykxhoorn, D.M., Palliser, D., Mizuno, H., Yu, E.Y., Eisen, H.N., Sabatini, D.M., Hahn, W.C., Sharp, P.A., Weinberg, R.A., et al. (2003). RNA 9, 493�501.
Note: There is no XhoI site in the shRNA oligo, as indicated on the author's map.
Proper citation: RRID:Addgene_10671 Copy
Species: Anabaena flos-aquae and Bacillus megaterium
Genetic Insert: ARG2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5374; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29300010
Proper citation: RRID:Addgene_106474 Copy
Species: Anabaena flos-aquae and Bacillus megaterium
Genetic Insert: ARG1
Vector Backbone Description: Backbone Marker:Gift from Jeff Hasty; Backbone Size:7293; Vector Backbone:pTD103; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29300010
Comments: Please note that some discrepancies were found between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_106475 Copy
Species: Homo sapiens
Genetic Insert: acetylcholinesterase
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18612832
Proper citation: RRID:Addgene_106442 Copy
Species: Mus musculus
Genetic Insert: osteopontin
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12944477
Comments: This plasmid is maintained at a medium copy number.
Proper citation: RRID:Addgene_106449 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:pX551; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29224783
Proper citation: RRID:Addgene_106431 Copy
Species: Synthetic
Genetic Insert: P-RAN-GFP1
Vector Backbone Description: Backbone Marker:Takara/Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29440485
Proper citation: RRID:Addgene_106408 Copy
Species: Synthetic
Genetic Insert: P-RAN-RFP6
Vector Backbone Description: Backbone Marker:Takara/Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29440485
Proper citation: RRID:Addgene_106411 Copy
Species: Synthetic
Genetic Insert: Rat Synaptobrevin-2 fragment 28-66
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28813412
Proper citation: RRID:Addgene_106414 Copy
Species: jellyfish
Genetic Insert: GFP shRNA
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15928077
Comments: shRNA oligo sequence: 5'-GCTACGTCCAGGAGCGCACCCTCGAGGGTGCGCTCCTGGACGTAGCC
Proper citation: RRID:Addgene_10675 Copy
Species: Homo sapiens
Genetic Insert: c-myc
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16024784
Proper citation: RRID:Addgene_10674 Copy
Species: Homo sapiens
Genetic Insert: PP2A B55 alpha shRNA
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14998489
Comments: To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 1156-1175 of PP2A B55 alpha followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.
Proper citation: RRID:Addgene_10679 Copy
Species: Homo sapiens
Genetic Insert: PP2A B56 alpha shRNA
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14998489
Comments: To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP).
Proper citation: RRID:Addgene_10680 Copy
Species: Homo sapiens
Genetic Insert: ALG14
Vector Backbone Description: Backbone Marker:Eric-Paul Bennett (Addgene plasmid # 68369); Backbone Size:2376; Vector Backbone:EPB104; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29315387
Proper citation: RRID:Addgene_106867 Copy
Species: Homo sapiens
Genetic Insert: human Importin beta-1 (KPNB1)
Vector Backbone Description: Backbone Marker: Clontech ; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15572412
Comments: This plasmid was created by Antonella Palena in the Lavia Lab.
Proper citation: RRID:Addgene_106941 Copy
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