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Species: Danio rerio
Genetic Insert: kcnmb3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164955 Copy
Species: Danio rerio
Genetic Insert: kcnn1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Comments: The DNA sequence was amplified from pooled wild-type TAB zebrafish embryos. According to the predicted sequence, XP_005171293.1, there are likely polymorphisms, K185E, H323Y, S599N, which are not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. In addition, at the 5’ end of the insert, there are 44bps extra forward primer dimer sequences (ATGCGGCATCGGCACGCGGGCCATGCGGCATCGGCACGCGGGCC) before the start codon. But this will not impact the result of the whole mount in situ hybridization.
Proper citation: RRID:Addgene_164956 Copy
Species: Danio rerio
Genetic Insert: kcnmb2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR Cloning Vectror; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164953 Copy
Species: Danio rerio
Genetic Insert: kcnn3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164959 Copy
Species: Danio rerio
Genetic Insert: kcnn1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164957 Copy
Species: Danio rerio
Genetic Insert: kcnn2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164958 Copy
Species: Danio rerio
Genetic Insert: kcnt1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Comments: The DNA sequence was amplified from pooled wild type TAB zebrafish embryos. There is a likely polymorphism, P17S, which is not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. But this will not impact the result of whole mount in situ hybridization
Proper citation: RRID:Addgene_164962 Copy
Species: Danio rerio
Genetic Insert: kcnt2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Comments: The DNA sequences were amplified from pooled wild type TAB zebrafish embryos. There are likely polymorphisms, N454D and D531E, which are not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. But this will not impact the result of whole-mount in situ hybridization
Proper citation: RRID:Addgene_164963 Copy
Species: Danio rerio
Genetic Insert: kcnn4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164960 Copy
Species: Danio rerio
Genetic Insert: kcnt1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164961 Copy
Species: Danio rerio
Genetic Insert: pax3a
Vector Backbone Description: Backbone Size:2784; Vector Backbone:p5E; Vector Types:Gateway 5 end entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32546498
Proper citation: RRID:Addgene_164966 Copy
Species: Homo sapiens
Genetic Insert: BACE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22553349
Proper citation: RRID:Addgene_165032 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
Proper citation: RRID:Addgene_16514 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: COV E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165123 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165088 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF3a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165121 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 ORF8
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165089 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF9b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165122 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp13 deltaN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165127 Copy
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