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Species: Synthetic
Genetic Insert: OphA-delta C6-P399A
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127689 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127679 Copy
Species: Synthetic
Genetic Insert: dCas9-CIBN-2A-Puro
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:3276; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127664 Copy
Species: Synthetic
Genetic Insert: CRY2-HA-2A-mCherry and gRNAs 115 and 117
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:4437; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127669 Copy
Species: Synthetic
Genetic Insert: CRY2-HA-2A-mCherry
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:3247; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127667 Copy
Species: Synthetic
Genetic Insert: gRNAs 129, 135, 115, 117
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:3308; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127666 Copy
Species: Synthetic
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:5582; Vector Backbone:Pmyo-3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952669
Proper citation: RRID:Addgene_127706 Copy
Species: Synthetic
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:3972; Vector Backbone:Pttr-39; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952669
Proper citation: RRID:Addgene_127704 Copy
Species: Synthetic
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:6564; Vector Backbone:Pacr-2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952669
Proper citation: RRID:Addgene_127703 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29564885
Comments: The insert mTq2-Tat was designed by G.Grossi and M. Schwarz-Schilling and cloned by A. Dupin and M. Schwarz-Schilling. mTq2 sequence is from Goedhart, J. et al. Nat. Commun. 2012, 3, 751. Tat sequence is from Bayer, T. S. et al. RNA 2005, 11, 1848−57. The peptide linker sequence between between YPet and PCP is TRGGGGS.
Proper citation: RRID:Addgene_127635 Copy
Species: Synthetic
Genetic Insert: RNA nanostrcutre
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29564885
Comments: The insert was designed by F. Chizzolini and M. Schwarz-Schilling and cloned by M.Schwarz-Schilling. The RNA sequence for the: 3WJ is from Shu, D. et al. Nucleic Acids Res. 2014, 42, e10; MG aptamer is from Babendure, J. R. et al. J. Am. Chem. Soc. 2003, 125, 14716−7; PP7 aptamer is from Chao, J. A. et al. Nat. Struct. Mol. Biol. 2008, 15, 103−5; TAR aptamer is from Bayer, T. S. et al. RNA 2005, 11, 1848−57; Streptavidin aptamer is from: Srisawat, C. and Engelke, D. R. RNA 2001, 7, 632−41.
Proper citation: RRID:Addgene_127634 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:1933; Vector Backbone:pMB1 zeocin; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_127638 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127729 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127728 Copy
Species: Synthetic
Genetic Insert: Synthetic CRISPR array containing four identical T. thermophilus spacers
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3920; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31278272
Proper citation: RRID:Addgene_127764 Copy
Species: Synthetic
Genetic Insert: Convergent LacI-TetR Toggle
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682205 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127761 Copy
Species: Synthetic
Genetic Insert: AsCpf1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31316073
Proper citation: RRID:Addgene_127750 Copy
Species: Synthetic
Genetic Insert: mSpinach,RFP Divergent Orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682222 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127756 Copy
Species: Synthetic
Genetic Insert: mSpinach, MG RNA aptamer in divergent orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682223 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127759 Copy
Species: Synthetic
Genetic Insert: mSpinach, MG RNA aptamer in convergent orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682223 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127758 Copy
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