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Species: Saccharomyces cerevisiae
Genetic Insert: PHO5pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164704 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
Vector Backbone Description: Vector Backbone:pRS410; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164849 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P(PGK1)-citrine and P(HIS4)-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pCR™Blunt II-TOPO®; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33303588
Comments: Digesting this plasmid with BciVI leaves compatible Gibson homology arms on either side of divergent promoter expression cassette, allowing direct Gibson assembly into the barcoded-gRNA plasmid library. See manuscript for additional details.
Proper citation: RRID:Addgene_164917 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L892W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1315 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (773-908)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4746; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1316 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GPD promoter-hsp82 ts mutant (G170D)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303 (based on pBluescript); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9371781
Comments: Plasmid maintained in DH5alpha.
Yeast hsp82 ts mutant expressed from the GPD promoter on a His3 marked integrating plasmid.
Proper citation: RRID:Addgene_1328 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Crz1*-CLASP
Vector Backbone Description: Vector Backbone:pYTK146; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32898473
Proper citation: RRID:Addgene_133085 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pCMK2-YFP
Vector Backbone Description: Vector Backbone:pYTK143; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32898473
Proper citation: RRID:Addgene_133119 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Act1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pPICZc; Vector Types:Yeast Expression, Pichia pastoris integration plasmid; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:29535210
Proper citation: RRID:Addgene_111148 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Proper citation: RRID:Addgene_111300 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Comments: Additional mutation in plasmid L206S- Not sure if this will affect plasmid function.
Proper citation: RRID:Addgene_111301 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP2
Vector Backbone Description: Backbone Size:7950; Vector Backbone:yCP50; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8722763
Comments: Full plasmid sequence contains several ambiguous bases in the backbone- Unsure if these regions will affect function.
Proper citation: RRID:Addgene_111304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8725222
Comments: Plasmid pJU225 (PRP8 TRPl2p) was constructed from
a previously described plasmid JDYl3 (GAL::PRP8) (BROWN
and BEGGS 1992). The galactose driven promoter in this plasmid
was removed by cutting with NheI and XhoI. A wild-type
copy of the promoter was amplified by PCR under nonmutagenic
conditions and used to replace the galactose driven
promoter. The entire PRP8 gene was then excised using XhoI
and NotI and ligated to plasmid pRS424 (TRPl 2p) also cut
with XhoI and NotI.
Proper citation: RRID:Addgene_111305 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8725222
Proper citation: RRID:Addgene_111307 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990037
Proper citation: RRID:Addgene_1113 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CWC25
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111346 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16103217
Comments: for use in in vitro splicing reactions
Cannot confirm del6- Please look at sequence before using.
Proper citation: RRID:Addgene_111331 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16103217
Comments: for use in in vitro splicing reactions
Cannot confirm del6- Please look at sequence before using.
Proper citation: RRID:Addgene_111332 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: snR7-L
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7585243
Comments: for in vitro transcription from T7 promoter
Proper citation: RRID:Addgene_111337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNR6
Vector Backbone Description: Backbone Marker:Stephen J. Elledge; Vector Backbone:pSE360; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2149118
Proper citation: RRID:Addgene_111281 Copy
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