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Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127746 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127745 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127744 Copy
Species: Synthetic
Genetic Insert: nourseothricin N-acetyltransferase
Vector Backbone Description: Vector Backbone:pDSM-ab; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: NatMX transcription unit with pathway position 2 homology arms, compatible with 5' position 1 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127747 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B4 VP1 gene
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32313222
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_127849 Copy
Species: Synthetic
Genetic Insert: 4 repeats of COX8 signal peptide plus GCaMP6f
Vector Backbone Description: Backbone Size:5433; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31862210
Proper citation: RRID:Addgene_127870 Copy
Species: Synthetic
Genetic Insert: Red-shifted mitochondrial matrix Ca2+ indicator
Vector Backbone Description: Backbone Size:5452; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31862210
Proper citation: RRID:Addgene_127873 Copy
Species: Synthetic
Genetic Insert: OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
Vector Backbone Description: Backbone Size:5394; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31862210
Proper citation: RRID:Addgene_127871 Copy
Species: Synthetic
Genetic Insert: 3xFLAG
Vector Backbone Description: Backbone Size:4578; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127862 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.N VP1 gene
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32313222
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_127851 Copy
Species: Synthetic
Genetic Insert: PDZ-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127961 Copy
Species: Synthetic
Genetic Insert: AAVS1-CAG-KRAB-dCas9-BFP-KRAB
Vector Backbone Description: Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127966 Copy
Species: Synthetic
Genetic Insert: Myr-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127963 Copy
Species: Synthetic
Genetic Insert: CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
Vector Backbone Description: Vector Backbone:pC13N; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127969 Copy
Species: Synthetic
Genetic Insert: AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
Vector Backbone Description: Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127967 Copy
Species: Synthetic
Genetic Insert: sYFP2-OP18-mScI-R125I
Vector Backbone Description: Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128002 Copy
Species: Synthetic
Genetic Insert: Myr-3xSAG-Dr-hel4-TrkB
Vector Backbone Description: Vector Backbone:pCMVd2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30850602
Comments: Please note that there are some discrepancies between Addgene's NGS sequence and the depositor's provided partial sequence. The depositor noted that these discrepancies do not affect plasmid function.
Proper citation: RRID:Addgene_127955 Copy
Species: Synthetic
Genetic Insert: ROPY-sYFP2(Y67G)
Vector Backbone Description: Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128008 Copy
Species: Synthetic
Genetic Insert: iGlucoSnFR-TS
Vector Backbone Description: Vector Backbone:pAAV.CAG...WPRE.SV40pA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31106909
Comments: Commercial use is protected by US Patent 9939437
Proper citation: RRID:Addgene_127952 Copy
Species: Synthetic
Genetic Insert: EB3-iRFP713
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128007 Copy
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