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Species: Synthetic
Genetic Insert: Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2
Vector Backbone Description: Backbone Size:8965; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353252
Comments: Please note: The plasmid contains a single A nucleotide deletion in the SARS-CoV-2 N gene ORF that causes a frameshift and early termination. This change is not expected to affect plasmid function as the only ORF on the plasmid is sfGFP (the RdRP and N gene fragments occur after the stop codon and are in the 3'UTR of sfGFP).
Proper citation: RRID:Addgene_155304 Copy
Species: Synthetic
Genetic Insert: 4E1H.95_LUMABS
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33398169
Proper citation: RRID:Addgene_155208 Copy
Species: Synthetic
Genetic Insert: 4E2H.210_LUMABS
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33398169
Proper citation: RRID:Addgene_155209 Copy
Species: Synthetic
Genetic Insert: 4E2H.210
Vector Backbone Description: Vector Backbone:pET11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398169
Proper citation: RRID:Addgene_155212 Copy
Species: Synthetic
Genetic Insert: iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155229 Copy
Species: Synthetic
Genetic Insert: H11-H4
Vector Backbone Description: Vector Backbone:pOPINO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32661423
Proper citation: RRID:Addgene_155364 Copy
Species: Synthetic
Genetic Insert: Nb H11-D4
Vector Backbone Description: Vector Backbone:pOPINO; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32661423
Proper citation: RRID:Addgene_155363 Copy
Species: Synthetic
Genetic Insert: PphlF-TA4 in dCas12a oscillator crRNAs
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155385 Copy
Species: Synthetic
Genetic Insert: dCas12a oscillator crRNAs
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155381 Copy
Species: Synthetic
Genetic Insert: PfEBA175
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32888502
Proper citation: RRID:Addgene_155312 Copy
Species: Synthetic
Genetic Insert: humanized EBpaRS/BstYam
Vector Backbone Description: Backbone Marker:Clontech, major modifications; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929862
Comments: The plasmid has been developed for efficient incorporation of Bpa in mammalian cells, first reported in Böttke et al, EMBO reports, 2020. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EBpaRS and 4 copies of the U6-BstYam tRNA cassette downstream of the synthetase cassette.
Proper citation: RRID:Addgene_155342 Copy
Species: Synthetic
Genetic Insert: 4X consensus BHLHE40 binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4983; Vector Backbone:pGL3 promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32205878
Proper citation: RRID:Addgene_153064 Copy
Species: Synthetic
Genetic Insert: eA3A-BE4 T31A-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_153000 Copy
Species: Synthetic
Genetic Insert: ABE7.10-nSpCas9
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152989 Copy
Species: Synthetic
Genetic Insert: eA3A-nSpCas9 NG-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152999 Copy
Species: Synthetic
Genetic Insert: eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152998 Copy
Species: Synthetic
Genetic Insert: eA3A-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152997 Copy
Species: Synthetic
Genetic Insert: BE4-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152991 Copy
Species: Synthetic
Genetic Insert: ABE7.10-nSpCas9 CP1041
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152990 Copy
Species: Synthetic
Genetic Insert: AID-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152994 Copy
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