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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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L4663 Resource Report Resource Website 1+ mentions |
RRID:Addgene_1665 | Ampicillin | See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD133.48, Ligation number L4663. | Backbone Size:6807; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:35 | 1 | ||||
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pCI-neo Flag HAUSP Resource Report Resource Website 10+ mentions |
RRID:Addgene_16655 | HAUSP | Homo sapiens | Ampicillin | PMID:15118411 | A fragment of human HAUSP cDNA including the entire ORF was cloned into pCI-neo at the NheI and XbaI sites. (Please note that the XbaI site is blocked by overlapping methylation). A sequence including the Flag tag was added to one of the primers so that the encoded protein contains a Flag tag at the N-terminus. | Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:36 | 18 | |
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Cyclin E-pBI-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_16654 | Cyclin E | Homo sapiens | Ampicillin | PMID:14999283 | A fragment of human cyclin E cDNA including the entire ORF was cloned into pBI-EGFP. | Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pBI-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:36 | 2 | |
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pCMV-Myc CDC4 R465C Resource Report Resource Website 1+ mentions |
RRID:Addgene_16653 | CDC4 R465C | Homo sapiens | Ampicillin | PMID:14999283 | A 1.5kb fragment of human CDC4 cDNA (nucleotides 374-1874), which encompasses the entire ORF, was cloned into pCMV-Myc. Addgene's quality control sequence suggests there are some unexpected mutations in the gene. The effect these mutations have on function is unknown, but this is the plasmid used in the Vogelstein article, so it should behave as reported. | Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R465C | 2026-08-15 01:09:36 | 2 |
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pRK172-α-syn-TEV-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_166671 | mouse synuclein (from V. Lee) | Mus musculus | Ampicillin | PMID:33497415 | The original plasmid reference is: "Selective imaging of internalized proteopathic a-synuclein seeds in primary neurons reveals mechanistic insight into transmission of synucleinopathies" JBC 2017, DOI 0.1074/jbc.M117.780296 Richard J. Karpowicz, Jr. , Conor M. Haney , Tiberiu S. Mihaila , Raizel M. Sandler , E. James Petersson, and Virginia M.-Y. Lee | Backbone Marker:Virginia Lee; Vector Backbone:pRK172; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | added a TEV cleavage site sequence between synuclein and GFP | 2026-08-15 01:09:37 | 2 |
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pAAV-TRE-DIO-eGFP-2A-TetanusToxin Resource Report Resource Website 1+ mentions |
RRID:Addgene_166603 | EGFP-2A-Tetanus Toxin | Synthetic | Ampicillin | PMID:33704065 | Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:36 | 3 | ||
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pGRG36 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16666 | mTn7::MCS | Ampicillin | PMID:16646962 | Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101. | Backbone Size:12549; Vector Backbone:pGRG36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:37 | 7 | ||
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pGRG25 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16665 | mTn7::MCS | Ampicillin | PMID:16646962 | Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101. | Backbone Size:12546; Vector Backbone:pGRG25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:36 | 2 | ||
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Venus-BimEL(Noxa)-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166761 | BimEL(Mus musculus)-(Noxa BH3) | Homo sapiens | Kanamycin | PMID:35442739 | BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | BH3 region removed and inserted BH3 of human Noxa protein | 2026-08-15 01:09:38 | 1 |
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pETDuet-LahT150 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166785 | LahT150 | Lachnospiraceae bacterium C6A11 | Ampicillin | PMID:30638446 | Backbone Marker:Addgene; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 | ||
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pETcon_SARS-CoV-2_RBD Resource Report Resource Website 1+ mentions |
RRID:Addgene_166782 | Spike receptor binding domain (RBD) from SARS-CoV-2 | Ampicillin | PMID:32841599 | Backbone Marker:Andrew Scharenberg; Vector Backbone:pETcon(-) (Plasmid #41522); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 6 | |||
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Venus-BimL-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166734 | BimL | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
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pLSB-NAT Resource Report Resource Website 1+ mentions |
RRID:Addgene_166698 | tRNA promoter : sgRNA cassette | Schizosaccharomyces pombe | Ampicillin | PMID:33313420 | The following acknowledgement must be included in all research publications and other outputs: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”. The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”. | Backbone Size:4988; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:37 | 2 | |
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Venus-Bik-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166737 | Bik | Homo sapiens | Kanamycin | PMID:35442739 | Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
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Venus-tBid-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166736 | tBid | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
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Venus-BimEL-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166735 | BimEL | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
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Venus-Puma-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166739 | Puma | Homo sapiens | Kanamycin | PMID:35442739 | Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 2 | |
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pAM-DCA-muGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_166727 | muGFP | synthetic/engineered | Ampicillin | PMID:29330459 | Backbone Size:5405; Vector Backbone:pAM-DCA; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:37 | 1 | ||
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mCerulean3-Mcl-1-pLVX Resource Report Resource Website 1+ mentions |
RRID:Addgene_166752 | Mcl-1 | Homo sapiens | Ampicillin | PMID:35442739 | 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins. | Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 | |
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mCerulean3-Bcl-W-FUGW Resource Report Resource Website 1+ mentions |
RRID:Addgene_166751 | Bcl-W | Mus musculus | Ampicillin | PMID:35442739 | 3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W. | Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 |
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