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Species: Saccharomyces cerevisiae
Genetic Insert: Cef1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234117 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The Ste3 promoter sequence (500 bprs upstream of the Ste3 ATG) was amplified from chromosomal DNA extracts using primers containing restriction enzyme adaptors for XhoI and XbaI. The pRS415-TEF1pr-Ste3-GFP plasmid was then digested with XhoI/XbaI to remove the TEF1 promoter and the Ste3 promoter sequences was ligated into its place.
Proper citation: RRID:Addgene_221112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE3-GFP
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The pRS415-TEF1pr plasmid (Mumberg et al., 1995) was used to insert the Ste3 coding sequence fused at the C-terminus to GFP. The GFP was inserted into the plasmid first between the XhoI and SalI restriction enzyme sites and then Ste3 coding sequence (lacking the stop codon) was inserted into the SpeI and SmaI restriction enzyme sites.
Proper citation: RRID:Addgene_221113 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234141 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234135 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234138 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Vector Backbone:pAG416-GAL-ccdb; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_181705 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prp8
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234124 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Syf1
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PrARG8-yeGFP
Vector Backbone Description: Backbone Marker:Irina Borodina Easy Clone 2.0 (Addgene #67296); Backbone Size:6049; Vector Backbone:pCfB2188; Vector Types:Yeast Expression, Cre/Lox, Integrative; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40172974
Comments: For further details refer to: EasyClone 2.0 Yeast Toolkit (Addgene Kit #1000000073 )
EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. Stovicek V, Borja GM, Forster J, Borodina I. J Ind Microbiol Biotechnol. 2015 Nov;42(11):1519-31. doi: 10.1007/s10295-015-1684-8. Epub 2015 Sep 16.
Proper citation: RRID:Addgene_216512 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC61-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of SEC61 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAPstar3
Vector Backbone Description: Backbone Size:5542; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39702652
Proper citation: RRID:Addgene_232740 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FAPoptim
Vector Backbone Description: Vector Backbone:pFA6-NATMX4; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: This FAPoptim integration plasmid was constructed by subcloning the FAPoptim sequence into the pFA6a-NATMX4 plasmid described in (Goldstein and McCusker, 1999). The resulting plasmid can be used for C-terminal tagging of endogenous protiens using the PCR-based integration strategy described in (Longtine et al., 1998).
Proper citation: RRID:Addgene_221129 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1-CUP1
Vector Backbone Description: Vector Backbone:pGAC24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234131 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1-CUP1
Vector Backbone Description: Vector Backbone:pGAC24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234130 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1-CUP1
Vector Backbone Description: Vector Backbone:pGAC24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234129 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pOP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Proper citation: RRID:Addgene_242501 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pOP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Proper citation: RRID:Addgene_242497 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pOP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Proper citation: RRID:Addgene_242496 Copy
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