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Species: Synthetic
Genetic Insert: MbPylRS-4ThzA
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253668 Copy
Species: Synthetic
Genetic Insert: chPylRS-4OxzA
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253663 Copy
Genetic Insert: Mb(IPYE)PylRS-τMH
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253665 Copy
Species: Synthetic
Genetic Insert: MmPylRS(8_2)
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253670 Copy
Species: Synthetic
Genetic Insert: chPylRS-2E5MH
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253657 Copy
Species: Synthetic
Genetic Insert: TLS2
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245882 Copy
Species: Synthetic
Genetic Insert: PtALSgRNA
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245881 Copy
Species: Synthetic
Genetic Insert: CsALS_gRNA1.1
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Golden Gate entry vector to expressing the 1st gRNA; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Proper citation: RRID:Addgene_245875 Copy
Species: Synthetic
Genetic Insert: TLS2
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245879 Copy
Species: Synthetic
Genetic Insert: CsALSgR1.1
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245877 Copy
Species: Synthetic
Genetic Insert: CsALSgR1.2
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Golden Gate entry vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Proper citation: RRID:Addgene_245876 Copy
Species: bacteria
Genetic Insert: RecA
Vector Backbone Description: Backbone Size:9700; Vector Backbone:Ts vector; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20885380
Proper citation: RRID:Addgene_249731 Copy
Genetic Insert: SceI
Vector Backbone Description: Vector Backbone:pSW-Tc; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36216579
Proper citation: RRID:Addgene_252220 Copy
Species: Streptococcus pasteurianus
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:miniCTX1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41670347
Proper citation: RRID:Addgene_253726 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205019 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205017 Copy
Species: Synthetic
Genetic Insert: OsU6.2-sgRNA7 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199733 Copy
Species: Synthetic
Genetic Insert: TaU6-sgRNA8 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199734 Copy
Species: Synthetic
Genetic Insert: ZmU6C3-sgRNA5 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199731 Copy
Species: Synthetic
Genetic Insert: ZmU6C1-sgRNA4 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199730 Copy
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