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Species: Danio rerio
Genetic Insert: Csf3b
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24128862
Proper citation: RRID:Addgene_168202 Copy
Species: Danio rerio
Genetic Insert: cdk2 sgRNAs
Vector Backbone Description: Vector Backbone:R4R3 pDest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168250 Copy
Species: Danio rerio
Genetic Insert: sp5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11641225
Comments: Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised.
Proper citation: RRID:Addgene_16884 Copy
Species: Danio rerio
Genetic Insert: 23f17
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Noncoding, repetitive transcript. Similar and overlapping with many other regions in the genome. For antisense probe synthesis.
Expressed in paraxial and ventral mesoderm during gastrulation. Later in specific brain regions.
For antisense: EcoRI, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16883 Copy
Species: Danio rerio
Genetic Insert: axin2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Zebrafish axin2 cDNA (unclear if full-length or not) in pSPORT1. Clone 24h4 from microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library). For antisense probe synthesis.
Pretty ubiquitously expressed during gastrulation. Positively regulated by Wnt signaling.
For antisense: Asp718, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16882 Copy
Species: Danio rerio
Genetic Insert: wnt8 5'orf2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: 588 bp PCR product ligated into BamHI and EcoRI cut pSK+.
For probe: cut with BamHI, use T7 RNA polymerase.
Proper citation: RRID:Addgene_16898 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker.
To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7.
Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_16897 Copy
Species: Danio rerio
Genetic Insert: Prickle-2
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2P+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Partial length prickle-2, contains the entire protein except the last 75 amino acids (predicted). Cloned into the BamH1 and Cla1 sites of CS2P+.
To make antisense probe: cut with BamH1, transcribe with T7. For the sense strand cut with Cla1 and transcribe with SP6.
Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_16896 Copy
Species: Danio rerio
Genetic Insert: Hrasls
Vector Backbone Description: Vector Backbone:Tol2-aACry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_168772 Copy
Species: Danio rerio
Genetic Insert: Dnase1l1l-mRFP
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_169002 Copy
Species: Danio rerio
Genetic Insert: vox partial
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Zvox24-1 cDNA from lambda ZAP vector. Encodes zebrafish partial vox gene. For in situs, cut with EcoRI, transcribe with T7.
Constructed by: David Kimelman, 7/2/96 XVII p.129.
Proper citation: RRID:Addgene_16901 Copy
Species: Danio rerio
Genetic Insert: Her5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For probe, digest with XhoI, transcribe with T3 polymerase.
Proper citation: RRID:Addgene_16908 Copy
Species: Danio rerio
Genetic Insert: BMP2b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Probe: cut with EcoRI, transcribe with T3 RNA polymerase.
Proper citation: RRID:Addgene_16915 Copy
Species: Danio rerio
Genetic Insert: snail
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T3.
Sense probe: cut with BamH1 and transcribe with T7.
Plasmid History: Constructed by Greg Kelly, 1/94.
Proper citation: RRID:Addgene_16931 Copy
Species: Danio rerio
Genetic Insert: eng2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2900; Vector Backbone:pGEM-4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T7.
Sense probe: cut with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Greg Kelly, 11/93.
Proper citation: RRID:Addgene_16930 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:SITS-ccdb-EGFP (Addgene 118752); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176019 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176013 Copy
Species: Danio rerio
Genetic Insert: Cavin4b (PRDmt)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176017 Copy
Species: Danio rerio
Genetic Insert: Cavin4a
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176010 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176008 Copy
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