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Species: Homo sapiens
Genetic Insert: Epac1 SAAX
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507992
Proper citation: RRID:Addgene_118313 Copy
Species: Homo sapiens
Genetic Insert: Epac1 CAAX
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507992
Proper citation: RRID:Addgene_118312 Copy
Species: Homo sapiens
Genetic Insert: RalGDS
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20547757
Proper citation: RRID:Addgene_118315 Copy
Species: Homo sapiens
Genetic Insert: PKI nls (aa 2-36)
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:peGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11390399
Comments: PKI constructs were subcloned into pEGFP containing Flag epitope with EcoRI and XbaI (see map).
Proper citation: RRID:Addgene_118301 Copy
Species: Homo sapiens
Genetic Insert: Caspase 9 catalytic mutant
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10085063
Proper citation: RRID:Addgene_11830 Copy
Species: Homo sapiens
Genetic Insert: CENP-A
Vector Backbone Description: Backbone Marker:MBL, Nagoya, Aichi, Japan; Backbone Size:4656; Vector Backbone:pmKO1-MC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18778791
Comments: Bgl II-KpnI fragment of pEGFP-AF8 (Addgene plasmid # 117803) was recloned into BamHI and KpnI site of pmKO1-MC1.
Note that the cloning strategy adds additional amino acids onto the C-terminus of the insert (STVPELQQRIRETREQSLNKRPRL), but this does not interfere with plasmid function. The extra sequence includes an MCS for adding tags to the C-terminus of the insert.
Proper citation: RRID:Addgene_118224 Copy
Species: Homo sapiens
Genetic Insert: Caspase 3
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11821 Copy
Species: Homo sapiens
Genetic Insert: Caspase 9
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10085063
Proper citation: RRID:Addgene_11829 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8 minus Death Effecter Domain catalytic mutant
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-15 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11828 Copy
Species: Homo sapiens
Genetic Insert: Caspase 6
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11823 Copy
Species: Homo sapiens
Genetic Insert: Caspase 7
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11825 Copy
Species: Homo sapiens
Genetic Insert: Craf S338A
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28694330
Comments: site directed mutagenesis
Proper citation: RRID:Addgene_118335 Copy
Species: Homo sapiens
Genetic Insert: Craf R401H
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28694330
Comments: site directed mutagenesis
Proper citation: RRID:Addgene_118334 Copy
Species: Homo sapiens
Genetic Insert: Craf R89L
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28694330
Proper citation: RRID:Addgene_118333 Copy
Species: Homo sapiens
Genetic Insert: Braf R188L
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28694330
Comments: site directed mutagenesis
Proper citation: RRID:Addgene_118338 Copy
Species: Homo sapiens
Genetic Insert: Rap2b
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20547757
Comments: human Rap2b was purchased from the Missouri S&T cDNA resouce center
Proper citation: RRID:Addgene_118320 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q
Vector Backbone Description: Backbone Size:0; Vector Backbone:p426 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10677504
Comments: N terminal region of Ht with polyQ repeat length of 103 fused to GFP.
Proper citation: RRID:Addgene_1184 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118379 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118378 Copy
Species: Homo sapiens
Genetic Insert: gRNA SORCS2 Human
Vector Backbone Description: Backbone Marker:Feng Zhang, Addgene plasmid #52961; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29868584
Proper citation: RRID:Addgene_118416 Copy
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