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Species: Homo sapiens
Genetic Insert: RINS1
Vector Backbone Description: Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28366620
Proper citation: RRID:Addgene_107290 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107320 Copy
Genetic Insert: RNA motif plasmid cloning backbone
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: Digest plasmid with BsmBI and ligate RNA motif of interest. The RNA motif of interest would be in the 3UTR and flanked by BoxB motifs.
Oligo cloning can be performed similarly to sgRNA cloning. Primers to order:
Primers (5'->3')
F GCTT
Proper citation: RRID:Addgene_107253 Copy
Species: Homo sapiens
Genetic Insert: 6x Halo-EGFP- 2x PACT
Vector Backbone Description: Vector Backbone:pERB219; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29097549
Proper citation: RRID:Addgene_107265 Copy
Species: Synthetic
Genetic Insert: ProA-RBS(B0032)-Gemini (E0051)
Vector Backbone Description: Backbone Marker:http://parts.igem.org/Part:pSB3C5; Backbone Size:2738; Vector Backbone:pSB3C5; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20843779
Proper citation: RRID:Addgene_107241 Copy
Genetic Insert: MARK3
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107235 Copy
Genetic Insert: toehold 7 + sfGFP
Vector Backbone Description: Backbone Marker:New England Biolabs (cloning vector); Backbone Size:2202; Vector Backbone:pNP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30083608
Proper citation: RRID:Addgene_107355 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: The BASU protein sequence has 4 mutations relative to wild type Bacillus subtilis biotin ligase (Uniprot P0CI75): N terminal truncation of 65 aa, R124G, E323S and G325R.
Proper citation: RRID:Addgene_107250 Copy
Species: Synthetic
Genetic Insert: Rncp-iGluSnFR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5268; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29308878
Comments: Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
Proper citation: RRID:Addgene_107336 Copy
Species: Homo sapiens
Genetic Insert: RB del663
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: del663 created by site-directed mutagenesis using this primer: GCCTATCTCCGGCTAaatgctgctatacgatcgCTTCTGTCTGAGCAC
Proper citation: RRID:Addgene_10728 Copy
Species: Homo sapiens
Genetic Insert: RB 567L
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: 567L created by site-directed mutagenesis using this primer: GCATGGCTCTCAGATcttCCTTTATTTGATCTT
Proper citation: RRID:Addgene_10725 Copy
Species: Homo sapiens
Genetic Insert: UBE3A
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWB-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28003368
Proper citation: RRID:Addgene_107349 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving PD-L1 under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124686 Copy
Species: Homo sapiens
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_124790 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving PD-1 under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124678 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving PD-1 under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124679 Copy
Species: Homo sapiens
Genetic Insert: MCP_ADAR2 DD (E488Q)_NLS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: Also expresses MS2_adRNA targeting the RAB7A transcript
Proper citation: RRID:Addgene_124705 Copy
Species: Homo sapiens
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_124788 Copy
Species: Synthetic
Genetic Insert: Venus-Akaluciferase
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman; Backbone Size:9780; Vector Backbone:pLenti PGK Neo DEST (Addgene #19067); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33241215
Proper citation: RRID:Addgene_124701 Copy
Vector Backbone Description: Vector Backbone:LRG2.1 (Addgene #108098); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31503414
Proper citation: RRID:Addgene_124770 Copy
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