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Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665592
Proper citation: RRID:Addgene_118363 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118357 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118359 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118358 Copy
Species: Homo sapiens
Genetic Insert: hGATA-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Proper citation: RRID:Addgene_118352 Copy
Species: Homo sapiens
Genetic Insert: hMEF2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Proper citation: RRID:Addgene_118355 Copy
Species: Homo sapiens
Genetic Insert: hMEF2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Proper citation: RRID:Addgene_118354 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12665592
Proper citation: RRID:Addgene_118360 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665592
Comments: Addgene NGS results found S2A within the SUMO1 translation compared to the closest NCBI reference (NP_003343.1). The depositing laboratory affirms that to their knowledge, this mutation does not change protein function
Proper citation: RRID:Addgene_118361 Copy
Species: Homo sapiens
Genetic Insert: hHSF2 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17213196
Proper citation: RRID:Addgene_118346 Copy
Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19229036
Proper citation: RRID:Addgene_118351 Copy
Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19229036
Proper citation: RRID:Addgene_118350 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens ceramide kinase
Vector Backbone Description: Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672528
Proper citation: RRID:Addgene_118600 Copy
Species: Homo sapiens
Genetic Insert: hPDL-1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pMP71; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30659053
Proper citation: RRID:Addgene_118631 Copy
Species: Homo sapiens
Genetic Insert: CD155
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pMP71; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30659053
Comments: The HA tag was removed to create an untagged ORF
Proper citation: RRID:Addgene_118630 Copy
Species: Homo sapiens
Genetic Insert: ERG (33-479)
Vector Backbone Description: Backbone Size:9500; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078722
Comments: TMPRSS2-ERG (aa33-479)
Proper citation: RRID:Addgene_118625 Copy
Species: Homo sapiens
Genetic Insert: ERG (R367K DNA binding mutant)
Vector Backbone Description: Vector Backbone:N106; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078722
Comments: V5 tagged TMPRSS2-ERG R367K (aa33-479) with 2aa linker between V5 and ERG
Proper citation: RRID:Addgene_118624 Copy
Species: Homo sapiens
Genetic Insert: ERG ( R367K DNA Binding mutant)
Vector Backbone Description: Backbone Size:9500; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078722
Comments: TMPRSS2-ERG R367K (aa33-479)
Proper citation: RRID:Addgene_118626 Copy
Species: Homo sapiens
Genetic Insert: ZFP64_Delta_TAD
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30503706
Proper citation: RRID:Addgene_118698 Copy
Species: Homo sapiens
Genetic Insert: Cadherin 5 (CD144)
Vector Backbone Description: Vector Backbone:pCSC-SP-PW-tdTomato; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: tdTomato cDNA was replaced with GFP in pCSC-SP-PW-GFP vector with Age I and Not I
I503T is a common SNP that does not affect function. Depositor confirms the expressed protein localizes to cadherin-based cell-cell junctions.
Proper citation: RRID:Addgene_118733 Copy
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