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Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118383 Copy
Species: Homo sapiens
Genetic Insert: XIAP K322/328R
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11834 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118380 Copy
Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665592
Proper citation: RRID:Addgene_118363 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118357 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118359 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118358 Copy
Species: Homo sapiens
Genetic Insert: hGATA-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Proper citation: RRID:Addgene_118352 Copy
Species: Homo sapiens
Genetic Insert: hMEF2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Proper citation: RRID:Addgene_118355 Copy
Species: Homo sapiens
Genetic Insert: hMEF2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Proper citation: RRID:Addgene_118354 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12665592
Proper citation: RRID:Addgene_118360 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665592
Comments: Addgene NGS results found S2A within the SUMO1 translation compared to the closest NCBI reference (NP_003343.1). The depositing laboratory affirms that to their knowledge, this mutation does not change protein function
Proper citation: RRID:Addgene_118361 Copy
Species: Homo sapiens
Genetic Insert: hHSF2 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17213196
Proper citation: RRID:Addgene_118346 Copy
Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19229036
Proper citation: RRID:Addgene_118351 Copy
Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19229036
Proper citation: RRID:Addgene_118350 Copy
Species: Homo sapiens
Genetic Insert: PBD HK-AA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/3x myc-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267267
Comments: Addgene plasmid #41162: Plk1-PBDWT (aa 326–603) was cloned in-frame by PCR into a pcDNA3.1 vector encoding an amino-terminal 3xmyc-tag (Invitrogen, Carlsbad, CA) using primers 5'-CCG GAA TTC CAG ATC TTC GAT TGC TCC CAG CAG CCT GG-3' and
5'-CCG CTC GAG TTA GGA GGC CTT GAG ACG GTT GC-3'.
The Plk1-PBDAA mutant (H538A, K540A) was made by site-directed mutagenesis using the following primers: 5'-CAA TTC TCC GGA GCC CCG CCT ATC TGT CCC-3' and 5'-GGG ACA GAT AGG CGG GGC TCC GGA GAA TTG-3'.
Proper citation: RRID:Addgene_41161 Copy
Species: Homo sapiens
Genetic Insert: Plk1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5527; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7962193
Comments: cDNA fragments spanning parts of the catalytic domains of human protein kinases were amplified by PCR, as described by Schultz and Nigg (1993). A 144 bp fragment (named HsPK28) showing 88% nucleotide identity to Drosophila polo was used to probe a λgt 10 library prepared from a human nasopharyngeal carcinoma (Hitt et al., 1989). Approximately 500,000 plaques were screened by plaque hybridization (Sambrook et al., 1989), and 17 phage showing strong hybridization were purified. DNA was prepared using Lambdasorb (Promega Biotech, Madison, WI).
One plasmid (referred to as Plk1-pGEM) with a 2143 bp insert contained the entire coding sequence for a protein kinase closely related to Drosophila polo. This insert was sequenced on both strands by the method of Chen and Seeburg (1985), using the Sequenase kit (United States Biochemicals).
To express a full-length Plk1 protein, the 1998 bp fragment of Plk1-pGEM was introduced into the pRc/CMV vector (Invitrogen Corporation, San Diego), to create Plk1-CMV. An N-terminally myc-tagged Plk1-CMV was also constructed. In the resulting mycplk1 protein, the N terminus of Plk1 is extended by the oligopeptide MEQKLISEEDLNMNSCSPGS (Evan et al., 1985).
Proper citation: RRID:Addgene_41160 Copy
Species: Homo sapiens
Genetic Insert: Rootletin
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4700; Vector Backbone:pCJW201 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16203858
Comments: The partial cDNA clones KIAA0445 (Kazusa DNA Research Institute) and IMAGE clone 6150861 (Deutsches Ressourcenzentrum fuer Genomforschung GmbH) were fused, and the complete coding sequence for rootletin was cloned into pBluescript II SK- (Addgene plasmid #41168) and subsequently subcloned into pEGFP-C1 (CLONTECH Laboratories, Inc.). All constructs were confirmed by sequencing.
Proper citation: RRID:Addgene_41166 Copy
Species: Homo sapiens
Genetic Insert: PICH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17218258
Comments: The complete ORF of PICH (corresponding exactly to FLJ20105; Acc. No. BC111486) was amplified by nested PCR, using a HeLa Marathon library (Clontech laboratories Inc.), and cloned into pEGFP-C1 vector (Clontech laboratories Inc.). The following primer pairs were used: primer pair 1: AAG CTC CAG CTC CAA GCT CC and TGC TTT TTG AGA TCT TTC TTG CC, primer pair 2: GACTCGAGCTATGGAGGCATCCCGAAGGTTTC and GCCCGGGTCAATTGTTATTAAGTTGC. These primers contain Xho1 and Xma1 sites, respectively, used for cloning.
Proper citation: RRID:Addgene_41163 Copy
Species: Homo sapiens
Genetic Insert: Cep215
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4110; Vector Backbone:pCJW206 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18042621
Comments: The Cep215 cDNA sequence was obtained from KIAA1633 clone (from Kazusa DNA Research Institute). Using PCR, a frameshift within the sequence was corrected and missing 5' coding information was obtained by PCR amplification from Marathon cDNA library (Clontech). Constructs were fused to yield the complete coding sequence of Cep215, and subcloned into a mammalian expression vector providing a Myc epitope-tag. All constructs were confirmed by sequencing.
Proper citation: RRID:Addgene_41152 Copy
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