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Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128214 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128213 Copy
Species: Synthetic
Genetic Insert: sgRNA#1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243081
Proper citation: RRID:Addgene_128179 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 5
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128218 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 4
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128217 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128173 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128171 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Proper citation: RRID:Addgene_128167 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: The depositor recommends the updated versions of this plasmid, PB CAG-sfGFP-T2A-iCreERT2 (https://www.addgene.org/177309/) or PB CAG-mTagBFP2-T2A-iCreERT2 (https://www.addgene.org/177308/).
Proper citation: RRID:Addgene_128168 Copy
Species: Synthetic
Genetic Insert: sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128201 Copy
Species: Synthetic
Genetic Insert: crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128208 Copy
Species: Synthetic
Genetic Insert: sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128206 Copy
Species: Synthetic
Genetic Insert: Acetamidase (amdS)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128363 Copy
Species: Synthetic
Genetic Insert: Acetamidase (amdS)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128362 Copy
Species: Synthetic
Genetic Insert: Acetamidase (amdS)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 1 sequence (CNAG_00777 - CNAG_00778) in the backbone of pBluescript II SK- flanked by blaster repeats to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128361 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27711143
Proper citation: RRID:Addgene_128354 Copy
Species: Synthetic
Genetic Insert: Noureothricin (NAT)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27711143
Comments: Please note: The plasmid contains a 390bp discrepancy in C-terminus of the LacZ Split. This region of the plasmid serves no purpose other than for screening and hosting the MCS used in generating the plasmid. This difference is not expected to affect the final function of this plasmid.
Proper citation: RRID:Addgene_128356 Copy
Species: Synthetic
Genetic Insert: Noureothricin (NAT)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27711143
Proper citation: RRID:Addgene_128355 Copy
Species: Synthetic
Genetic Insert: Acetamidase (amdS)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the amdS2 mCherry Blaster marker with a flexable linker in the multicloning site of pBluescript II SK-. Importantly, the amdS2 marker can be excised using restriction enzymes HindIII and EcoRI.
Proper citation: RRID:Addgene_128359 Copy
Species: Synthetic
Genetic Insert: TetR-LOV-TIP
Vector Backbone Description: Vector Backbone:pMTG05-Amp-FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31269201
Comments: Additional relevant information can be found from this publication: Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471
Proper citation: RRID:Addgene_128277 Copy
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