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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pENTR-actin-V159N Resource Report Resource Website |
RRID:Addgene_118383 | actin beta | Homo sapiens | Kanamycin | PMID:30890647 | Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint | Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin | V159N | 2026-08-15 01:03:03 | 0 |
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pcDNA3-Xiap-Myc K322/328R Resource Report Resource Website 1+ mentions |
RRID:Addgene_11834 | XIAP K322/328R | Homo sapiens | Ampicillin | Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K322/328R | 2026-08-15 01:03:03 | 1 | ||
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pENTR-NLS-actin Resource Report Resource Website |
RRID:Addgene_118380 | actin beta | Homo sapiens | Kanamycin | PMID:30890647 | Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint | Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin | NLS | 2026-08-15 01:03:03 | 0 |
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hHSF1 S303A Resource Report Resource Website |
RRID:Addgene_118363 | hHSF1 | Homo sapiens | Ampicillin | PMID:12665592 | Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S303A | 2026-08-15 01:03:02 | 0 | |
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hHSF4b K294R Resource Report Resource Website |
RRID:Addgene_118357 | hHSF4b | Homo sapiens | Ampicillin | PMID:16371476 | Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K294R | 2026-08-15 01:03:02 | 0 |
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Gal4-HSF4b K294R Resource Report Resource Website |
RRID:Addgene_118359 | hHSF4b | Homo sapiens | Ampicillin | PMID:16371476 | Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes. | Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K294R | 2026-08-15 01:03:02 | 0 |
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Gal4-HSF4b WT Resource Report Resource Website |
RRID:Addgene_118358 | hHSF4b | Homo sapiens | Ampicillin | PMID:16371476 | Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes. | Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:03:03 | 0 |
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hGATA-1 WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_118352 | hGATA-1 | Homo sapiens | Ampicillin | PMID:16371476 | Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:03:03 | 2 | |
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hMEF2A K403R Resource Report Resource Website |
RRID:Addgene_118355 | hMEF2A | Homo sapiens | Ampicillin | PMID:16371476 | Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K403R | 2026-08-15 01:03:03 | 0 | |
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hMEF2A WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_118354 | hMEF2A | Homo sapiens | Ampicillin | PMID:16371476 | Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:03:02 | 2 | |
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hSUMO1 GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_118360 | hSUMO1 | Homo sapiens | Kanamycin | PMID:12665592 | Backbone Marker:Clonetech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | WT | 2026-08-15 01:03:02 | 1 | |
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hSUMO1-HIS Resource Report Resource Website 1+ mentions |
RRID:Addgene_118361 | hSUMO1 | Homo sapiens | Ampicillin | PMID:12665592 | Addgene NGS results found S2A within the SUMO1 translation compared to the closest NCBI reference (NP_003343.1). The depositing laboratory affirms that to their knowledge, this mutation does not change protein function | Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Please see Depositor Comments | 2026-08-15 01:03:03 | 1 |
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pSUPER-hHSF2 shRNA Resource Report Resource Website |
RRID:Addgene_118346 | hHSF2 shRNA | Homo sapiens | Ampicillin | PMID:17213196 | Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:03:02 | 0 | |
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hHSF1 K80Q Resource Report Resource Website |
RRID:Addgene_118351 | hHSF1 | Homo sapiens | Ampicillin | PMID:19229036 | Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K80Q | 2026-08-15 01:03:02 | 0 | |
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hHSF1 K80R Resource Report Resource Website |
RRID:Addgene_118350 | hHSF1 | Homo sapiens | Ampicillin | PMID:19229036 | Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K80R | 2026-08-15 01:03:02 | 0 | |
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pcDNA3 PBD HK-AA (Nigg AH13) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41161 | PBD HK-AA | Homo sapiens | Ampicillin | PMID:16267267 | Addgene plasmid #41162: Plk1-PBDWT (aa 326–603) was cloned in-frame by PCR into a pcDNA3.1 vector encoding an amino-terminal 3xmyc-tag (Invitrogen, Carlsbad, CA) using primers 5'-CCG GAA TTC CAG ATC TTC GAT TGC TCC CAG CAG CCT GG-3' and 5'-CCG CTC GAG TTA GGA GGC CTT GAG ACG GTT GC-3'. The Plk1-PBDAA mutant (H538A, K540A) was made by site-directed mutagenesis using the following primers: 5'-CAA TTC TCC GGA GCC CCG CCT ATC TGT CCC-3' and 5'-GGG ACA GAT AGG CGG GGC TCC GGA GAA TTG-3'. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/3x myc-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Contains Plk1-PBD only (aa 326–603); HK-AA (H538A, K540A) | 2026-08-15 01:14:42 | 1 |
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pRcCMV myc-Plk1 wt (Nigg RG6) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41160 | Plk1 | Homo sapiens | Ampicillin | PMID:7962193 | cDNA fragments spanning parts of the catalytic domains of human protein kinases were amplified by PCR, as described by Schultz and Nigg (1993). A 144 bp fragment (named HsPK28) showing 88% nucleotide identity to Drosophila polo was used to probe a λgt 10 library prepared from a human nasopharyngeal carcinoma (Hitt et al., 1989). Approximately 500,000 plaques were screened by plaque hybridization (Sambrook et al., 1989), and 17 phage showing strong hybridization were purified. DNA was prepared using Lambdasorb (Promega Biotech, Madison, WI). One plasmid (referred to as Plk1-pGEM) with a 2143 bp insert contained the entire coding sequence for a protein kinase closely related to Drosophila polo. This insert was sequenced on both strands by the method of Chen and Seeburg (1985), using the Sequenase kit (United States Biochemicals). To express a full-length Plk1 protein, the 1998 bp fragment of Plk1-pGEM was introduced into the pRc/CMV vector (Invitrogen Corporation, San Diego), to create Plk1-CMV. An N-terminally myc-tagged Plk1-CMV was also constructed. In the resulting mycplk1 protein, the N terminus of Plk1 is extended by the oligopeptide MEQKLISEEDLNMNSCSPGS (Evan et al., 1985). | Backbone Marker:Invitrogen; Backbone Size:5527; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:42 | 5 | |
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pEGFP Rootletin (Nigg pFL2(CW499)) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41166 | Rootletin | Homo sapiens | Kanamycin | PMID:16203858 | The partial cDNA clones KIAA0445 (Kazusa DNA Research Institute) and IMAGE clone 6150861 (Deutsches Ressourcenzentrum fuer Genomforschung GmbH) were fused, and the complete coding sequence for rootletin was cloned into pBluescript II SK- (Addgene plasmid #41168) and subsequently subcloned into pEGFP-C1 (CLONTECH Laboratories, Inc.). All constructs were confirmed by sequencing. | Backbone Marker:Modified from Clontech; Backbone Size:4700; Vector Backbone:pCJW201 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:42 | 5 | |
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pEGFP PICH (Nigg CB62) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41163 | PICH | Homo sapiens | Kanamycin | PMID:17218258 | The complete ORF of PICH (corresponding exactly to FLJ20105; Acc. No. BC111486) was amplified by nested PCR, using a HeLa Marathon library (Clontech laboratories Inc.), and cloned into pEGFP-C1 vector (Clontech laboratories Inc.). The following primer pairs were used: primer pair 1: AAG CTC CAG CTC CAA GCT CC and TGC TTT TTG AGA TCT TTC TTG CC, primer pair 2: GACTCGAGCTATGGAGGCATCCCGAAGGTTTC and GCCCGGGTCAATTGTTATTAAGTTGC. These primers contain Xho1 and Xma1 sites, respectively, used for cloning. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:42 | 5 | |
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pRcCMV Cep215 (Nigg CW493) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41152 | Cep215 | Homo sapiens | Kanamycin | PMID:18042621 | The Cep215 cDNA sequence was obtained from KIAA1633 clone (from Kazusa DNA Research Institute). Using PCR, a frameshift within the sequence was corrected and missing 5' coding information was obtained by PCR amplification from Marathon cDNA library (Clontech). Constructs were fused to yield the complete coding sequence of Cep215, and subcloned into a mammalian expression vector providing a Myc epitope-tag. All constructs were confirmed by sequencing. | Backbone Marker:Modified from Clontech; Backbone Size:4110; Vector Backbone:pCJW206 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:42 | 4 |
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