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pFH71
 
Resource Report
Resource Website 		RRID:Addgene_128214 improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003) Synthetic Kanamycin PMID:32326901 Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pFH51
 
Resource Report
Resource Website 		RRID:Addgene_128213 improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38) Synthetic Kanamycin PMID:32326901 Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pBM019
 
Resource Report
Resource Website 		RRID:Addgene_128179 sgRNA#1 Synthetic Ampicillin PMID:31243081 Backbone Size:4000; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:44 0
pAK005
 
Resource Report
Resource Website 		RRID:Addgene_128218 improved sgRNA backbone for tRNA-gRNA array, Position 5 Synthetic Kanamycin PMID:32326901 Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pAK004
 
Resource Report
Resource Website 		RRID:Addgene_128217 improved sgRNA backbone for tRNA-gRNA array, Position 4 Synthetic Kanamycin PMID:32326901 Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pCAGGS-mTagBFP2-T2A-iCreERT2 (PVGSA)
 
Resource Report
Resource Website 		RRID:Addgene_128173 mTagBFP2-T2A-iCreERT2 Synthetic Ampicillin Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:04:44 0
pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)
 
Resource Report
Resource Website 		RRID:Addgene_128171 mTagBFP2-T2A-iCreERT2 Synthetic Ampicillin Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:04:44 0
pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
 
Resource Report
Resource Website 		RRID:Addgene_128167 mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2 Synthetic Ampicillin PMID:32183906 Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749). The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry. Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:04:44 0
pCAGGS-mTagBFP2-T2A-iCre-ERT2
 
Resource Report
Resource Website 		RRID:Addgene_128168 mTagBFP2-T2A-iCreERT2 Synthetic Ampicillin PMID:32183906 The depositor recommends the updated versions of this plasmid, PB CAG-sfGFP-T2A-iCreERT2 (https://www.addgene.org/177309/) or PB CAG-mTagBFP2-T2A-iCreERT2 (https://www.addgene.org/177308/). Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:04:44 0
pFH88
 
Resource Report
Resource Website 		RRID:Addgene_128201 sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33) Synthetic Kanamycin PMID:32326901 Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pFH103
 
Resource Report
Resource Website 		RRID:Addgene_128208 crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35) Synthetic Kanamycin PMID:32326901 Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pFH101
 
Resource Report
Resource Website 		RRID:Addgene_128206 sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35) Synthetic Kanamycin PMID:32326901 Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:44 0
pPEE20
 
Resource Report
Resource Website 		RRID:Addgene_128363 Acetamidase (amdS) Synthetic Ampicillin PMID:31220607 Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pPEE19
 
Resource Report
Resource Website 		RRID:Addgene_128362 Acetamidase (amdS) Synthetic Ampicillin PMID:31220607 Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pPEE17
 
Resource Report
Resource Website 		RRID:Addgene_128361 Acetamidase (amdS) Synthetic Ampicillin PMID:31220607 Contains the Safe Haven Site 1 sequence (CNAG_00777 - CNAG_00778) in the backbone of pBluescript II SK- flanked by blaster repeats to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pSDMA65
 
Resource Report
Resource Website 		RRID:Addgene_128354 Hygromycin (HYG) Synthetic Ampicillin PMID:27711143 Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pSDMA67
 
Resource Report
Resource Website 		RRID:Addgene_128356 Noureothricin (NAT) Synthetic Ampicillin PMID:27711143 Please note: The plasmid contains a 390bp discrepancy in C-terminus of the LacZ Split. This region of the plasmid serves no purpose other than for screening and hosting the MCS used in generating the plasmid. This difference is not expected to affect the final function of this plasmid. Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pSDMA66
 
Resource Report
Resource Website 		RRID:Addgene_128355 Noureothricin (NAT) Synthetic Ampicillin PMID:27711143 Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pPEE13
 
Resource Report
Resource Website 		RRID:Addgene_128359 Acetamidase (amdS) Synthetic Ampicillin PMID:31220607 Contains the amdS2 mCherry Blaster marker with a flexable linker in the multicloning site of pBluescript II SK-. Importantly, the amdS2 marker can be excised using restriction enzymes HindIII and EcoRI. Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:04:46 0
pMTG8-Amp-FRT
 
Resource Report
Resource Website 		RRID:Addgene_128277 TetR-LOV-TIP Synthetic Ampicillin PMID:31269201 Additional relevant information can be found from this publication: Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471 Vector Backbone:pMTG05-Amp-FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:04:45 0

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