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Species: Homo sapiens
Genetic Insert: S100P
Vector Backbone Description: Backbone Size:9414; Vector Backbone:pLAS3w; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30967631
Proper citation: RRID:Addgene_125009 Copy
Species: Caenorhabditis elegans
Genetic Insert: RGG-GFP-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124948 Copy
Species: Caenorhabditis elegans
Genetic Insert: RGG-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124941 Copy
Species: Caenorhabditis elegans
Genetic Insert: RGG-GFP-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124939 Copy
Species: Caenorhabditis elegans
Genetic Insert: RGG-GFP-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124930 Copy
Species: Caenorhabditis elegans
Genetic Insert: RGG-RFP-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124934 Copy
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125134 Copy
Species: Other
Genetic Insert: GDH2 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125043 Copy
Vector Backbone Description: Backbone Size:6926; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that Halotag can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125138 Copy
Species: Tobacco etch virus
Genetic Insert: TEV_SH
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pTH24 based on pDEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16150509
Comments: Please note that this plasmid may run a dimer (>13kb) or multimer.
Proper citation: RRID:Addgene_125194 Copy
Species: Homo sapiens
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:origene; Backbone Size:7600; Vector Backbone:pLenti-c-Myc-DDK-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25795775
Proper citation: RRID:Addgene_125238 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid #1764); Backbone Size:5150; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16339315
Comments: To construct a hemagglutinin (HA)-tagged allele of p110 alpha, a cDNA clone of human PIK3CA (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics [HIP]) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.
Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.
Proper citation: RRID:Addgene_12522 Copy
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pAdTrace-TO4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This is an RFP expressing adenoviral vector compatible with the AdEasy system. The mRFP is functional and the depositing lab has been using it as a control. The stop codon was originally deleted in order to make c-terminal fusion proteins. But the current construct should run into certain stop codons at the end of the linker sites.
Proper citation: RRID:Addgene_12520 Copy
Species: Homo sapiens
Genetic Insert: Polycomb protein EED
Vector Backbone Description: Backbone Marker:a gift from the lab of Dr. Yufeng Tong, University of Toronto, Addgene #62304; Backbone Size:6767; Vector Backbone:pFBOH-MHL baculovirus expression vector; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125174 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Size:5986; Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression, baculovirus expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125161 Copy
Species: Homo sapiens
Genetic Insert: PHF19
Vector Backbone Description: Backbone Size:5986; Vector Backbone:pFB1.HMBP.A3.PrS.ybbR (parent pFast.Bac1); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125168 Copy
Species: Homo sapiens
Genetic Insert: MTF2
Vector Backbone Description: Backbone Size:5986; Vector Backbone:pFB1.HMBP.A3.PrS.ybbR (parent pFast.Bac1); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125169 Copy
Vector Backbone Description: Backbone Size:3228; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12019795
Proper citation: RRID:Addgene_12514 Copy
Species: Mus musculus
Genetic Insert: CCN1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pAdTrace; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16581771
Comments: pAdTrace is an RFP-based shuttle vector.
Proper citation: RRID:Addgene_12519 Copy
Species: Mus musculus
Genetic Insert: Wnt3A
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15496414
Proper citation: RRID:Addgene_12518 Copy
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