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Species: Homo sapiens
Genetic Insert: Cep170
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4700; Vector Backbone:pEGFP-T7/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15616186
Comments: The KIAA0470 cDNA was obtained from the Kazusa DNA Research Institute. It was subcloned into pT7-EGFP-C1 (modified from pEGFP-C1; BD Biosciences Clontech), after removing the ATG. Specifically, a 5kb SalI-SnaBI (Klenow blunted) fragment from pBS/KIAA0470Δ5'UTR was ligated into pT7-EGFP-C1 (Nigg COM81) digested with XhoI (Klenow blunted). The 3' XhoI site was recreated. This resulted in Addgene plasmid #41150: pEGFP Cep170 (Nigg PID200). The plasmid was then digested with BspEI and XmnI, blunted, and religated to recover a plasmid containing only the Cep170 C-terminal portion (aa 755-1460, from XmnI site to the end of cDNA).
Proper citation: RRID:Addgene_41151 Copy
Species: Homo sapiens
Genetic Insert: RIP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19524513
Proper citation: RRID:Addgene_41389 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Proper citation: RRID:Addgene_41569 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Comments: 5' cloning site: HindIII
3' cloning site: NotI
Proper citation: RRID:Addgene_41567 Copy
Species: Homo sapiens
Genetic Insert: SIRT6
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3PKI/
Proper citation: RRID:Addgene_41565 Copy
Species: Homo sapiens
Genetic Insert: PREX2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19729658
Proper citation: RRID:Addgene_41555 Copy
Species: Homo sapiens
Genetic Insert: MAL
Vector Backbone Description: Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Comments: The depositing lab generated pEF-Bos MAL Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The depositor's provided sequence includes some flanking EF-BOS sequence. The construct also contains a HIS tag.
Proper citation: RRID:Addgene_41554 Copy
Species: Homo sapiens
Genetic Insert: ASC
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19158675
Proper citation: RRID:Addgene_41553 Copy
Species: Homo sapiens
Genetic Insert: TRAM
Vector Backbone Description: Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14517278
Comments: The depositing lab generated pEF-Bos Tram Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The construct also contains a HIS tag.
Proper citation: RRID:Addgene_41551 Copy
Species: Homo sapiens
Genetic Insert: glucose-6-phosphate dehydrogenase
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21336310
Proper citation: RRID:Addgene_41521 Copy
Species: Homo sapiens
Genetic Insert: Transmembrane Protein 216
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22282472
Comments: See Table S6 of Supporting Online Material from associated publication for TMEM216 reference sequence.
Proper citation: RRID:Addgene_41633 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41590 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41589 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41587 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41586 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41584 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Proper citation: RRID:Addgene_41580 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Proper citation: RRID:Addgene_41579 Copy
Species: Homo sapiens
Genetic Insert: hPXR-LBD-T248E
Vector Backbone Description: Backbone Marker:ATCC (Item #77476); Backbone Size:5900; Vector Backbone:pSH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Human PXR ligand binding domain (LBD, aa 107-434) was cloned into vector pSH. Mutations were made and confirmed by sequencing.
Proper citation: RRID:Addgene_41619 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Comments: 5' cloning site: EcoR1
3' cloning site: XhoI
Proper citation: RRID:Addgene_41575 Copy
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