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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Z-FUS-5
 
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RRID:Addgene_166561 single chain-Fv, scFv Homo sapiens Kanamycin Target NCBI gene ID: 2521 Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:36 0
Z-AIMP1-1
 
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RRID:Addgene_166560 single chain-Fv, scFv Homo sapiens Kanamycin Target NCBI gene ID: 9255 Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:36 0
G-BRD4-4
 
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RRID:Addgene_166518 single chain-Fv, scFv Homo sapiens Kanamycin Target NCBI gene ID: 23476 Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:36 0
G-SMARCA4-2
 
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RRID:Addgene_166519 single chain-Fv, scFv Homo sapiens Kanamycin Target NCBI gene ID: 6597 Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:36 0
Venus-79aa-ActA-pEGFP-C1
 
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RRID:Addgene_166763 ActA tail anchor (Listeria monocytogenes) Synthetic Kanamycin "SGLRSRGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDAR GSGLRSRAQASNSAVDARV" amino acid targeting sequence, which we refer to as "ActA", for the mitochondrial outer membrane. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "79aa" indicates the 79 amino acid flexible linker region between Venus and the ActA targeting signal. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:38 0
Venus-30aa-Noxa-pEGFP-C1
 
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RRID:Addgene_166762 Noxa Homo sapiens Kanamycin Venus fused to the N-terminus of human Noxa amino acid sequence: "MPGKKARKNAQPSPARAPAELEVECATQLRRFGDKLNFRQKLLNLISKLFCSGT". Linked by a 30 amino acid (aa) linker. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:38 0
Venus-BimEL(Noxa)-pEGFP-C1
 
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RRID:Addgene_166761 BimEL(Mus musculus)-(Noxa BH3) Homo sapiens Kanamycin PMID:35442739 BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin BH3 region removed and inserted BH3 of human Noxa protein 2026-08-15 01:09:38 1
Venus-7aa-MAO-pEGFP-C3
 
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RRID:Addgene_166765 MAO tail anchor Homo sapiens Kanamycin "WERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS" amino acid targeting sequence, which we refer to as "MAO", for mitochondrial outer membrane targeting. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "7aa" indicates the 7 amino acid flexible linker region between Venus and the ActA targeting signal for mitochondria. Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:38 0
Venus-7aa-Cb5-pEGFP-C1
 
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RRID:Addgene_166764 Cb5 tail anchor Rattus norvegicus Kanamycin "KITTVESNSSWWTNWVIPAISALVVALMYRLYMAED" amino acid targeting sequence, which we refer to as "Cb5", for endoplasmic reticulum targeting. "7aa" indicates the 7 amino acid flexible linker region between Venus and the Cb5 targeting signal. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:38 0
pCA528-PPxY1-GS-WW3e
 
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RRID:Addgene_166705 AMOT PPxY1 fused to NEDD4L WW3 Homo sapiens Kanamycin PMID:34284061 Backbone Size:5597; Vector Backbone:pCA528; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 0
Venus-BimL-pEGFP-C1
 
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1+ mentions
RRID:Addgene_166734 BimL Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-Bik-pEGFP-C1
 
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RRID:Addgene_166737 Bik Homo sapiens Kanamycin PMID:35442739 Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-tBid-pEGFP-C1
 
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1+ mentions
RRID:Addgene_166736 tBid Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-BimEL-pEGFP-C1
 
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1+ mentions
RRID:Addgene_166735 BimEL Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-Puma-pEGFP-C1
 
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RRID:Addgene_166739 Puma Homo sapiens Kanamycin PMID:35442739 Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 2
Venus-Bad-4E-pEGFP-C1
 
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1+ mentions
RRID:Addgene_166740 Bad-4E Homo sapiens Kanamycin PMID:30860026 Transfection of this plasmid will express "VBad-4E" (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 4 hydrophobic residues in the BH3 region mutated to glutamic acid: Y110E, L114E, M117E, F122E. 2026-08-15 01:09:37 1
Venus-tBid-4E-pEGFP-C1
 
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1+ mentions
RRID:Addgene_166743 tBid-4E Mus musculus Kanamycin PMID:35442739 Transfection of this plasmid will express "VtBid-4E" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E). BH3, Mutations: I27E, L31E, I34E, M38E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 4 hydrophobic residues in the BH3 region mutated to glutamic acid: I27E, L31E, I34E, M38E. 2026-08-15 01:09:37 1
Venus-BimEL-4E-pEGFP-C1
 
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1+ mentions
RRID:Addgene_166742 BimEL-4E Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VBimEL-4E" (Venus fused to the N-terminus of the BH3-only protein, BimEL-4E) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL-4E proteins. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: I146E, L150E, I153E, F157E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 4 hydrophobic residues in the BH3 region mutated to glutamic acid: I146E, L150E, I153E, F157E. 2026-08-15 01:09:37 1
mILK-R255A-EFGP
 
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RRID:Addgene_176897 Integrin-linked kinase Mus musculus Kanamycin PMID:35259013 Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437490v1 for bioRxiv preprint. Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Arginine 255 to Alanine 2026-08-15 01:11:02 0
RNT1-GFP
 
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1+ mentions
RRID:Addgene_17708 rent1 Homo sapiens Kanamycin PMID:12228722 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:11:05 5

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