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Species: Other
Genetic Insert: CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36088516
Comments: L2 4X opattB late iridiod cytosol v2: P1_4X opattB-TMV- CrSTR-35Sterm + P2_4X opattB-TMV-Cr7DLH-masterm + P3_4X opattB-TMV-CrLAMT-ocsterm + P4_4X opattB-TMV-CrTDC-g7term + P5_4X opattB-TMV-CrSLS-nosterm
Proper citation: RRID:Addgene_177091 Copy
Species: Other
Genetic Insert: CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36088516
Comments: L2 4X opattB middle iridiod cytosol v3: P1_4X opattB-TMV-CrISY-35Sterm + P2_4X opattB-TMV-NmMLP-masterm + P3_4X opattB-TMV-Cr7DLGT-nosterm + P4_4X opattB-TMV-Cr8HGO/GOR-g7term + P5_4X opattB-TMV-CrIO-35Sterm + P6_4X opattB-TMV-CrG8H-ocsterm
Proper citation: RRID:Addgene_177090 Copy
Species: Other
Genetic Insert: P19, CrDXS2, PaGPPS1, and CrGES
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36088516
Comments: L2 P19 + 4X opattB early iridiod chloroplast v6: P1_nosP-TMV-P19-nosterm + P2_4X opattB-TMV-cTP_CrDXS2-masterm + P3_4X opattB-TMV-cTP_PaGPPS1-ocsterm + P4_4X opattB-TMV-cTP_CrGES-g7term)
Proper citation: RRID:Addgene_177088 Copy
Genetic Insert: MG1655 PlacZYA::Plac-gfp-muNS-kan
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24361104
Comments: GFP-muNS expression is under the control of Plac promoter integrated into the endogenous lac locus. The native lac operon (lacZYA) is deleted. Kanamycin resistance casette is inserted downstream of the gfp-muNS gene for cloning purposes.
Cloning method: Lambda RED recombination. The gfp-μNS fragment was amplified from plasmid pER12 (pBAD322A-gfp-μNS), which encodes an N-terminal GFP fusion to residues 471-721 of μNS (Broering et al., 2005) and then digested by KpnI/XhoI. The nptI gene was amplified from pKD4 plasmid and digested by XhoI/SmaI. Then, gfp-μNS and nptI gene fragments were triple ligated into the pKS plasmid. The resulting plasmid was used as a PCR template to generate a gfp-μNS-nptI fragment with flanking regions complementary to lacI and cynX, respectively. This fragment was then used to replace the lac operon (lacZYA) by one-step λ-recombination (Datsenko and Wanner, 2000). The resulting gfp-μNS-nptI replacement of lacZYA was transduced back into MG1655 by P1-phage to generate strain CJW4617. See Parry et al., 2014 for more details. This strain doesn't contain any plasmid.
Proper citation: RRID:Addgene_177118 Copy
Species: Mus musculus
Genetic Insert: STIM1
Vector Backbone Description: Backbone Size:2200; Vector Backbone:Modified pENTR11; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18327260
Comments: Mouse Stim1 (BglII/NotI) was subcloned into BglII/EagI followed by a myc tag.
Proper citation: RRID:Addgene_17733 Copy
Species: Mus musculus
Genetic Insert: STIM2
Vector Backbone Description: Backbone Size:2200; Vector Backbone:Modified pENTR11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18327260
Proper citation: RRID:Addgene_17734 Copy
Species: Methanogenic archaeon ISO4-G1
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:2452; Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35005899
Proper citation: RRID:Addgene_177310 Copy
Species: Methanogenic archaeon ISO4-G1
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:2452; Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34780162
Proper citation: RRID:Addgene_177311 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34614191
Proper citation: RRID:Addgene_177666 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34614191
Proper citation: RRID:Addgene_177667 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177711 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1-like 2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19898420
Proper citation: RRID:Addgene_177842 Copy
Species: Homo sapiens
Genetic Insert: AMPK (a1b2g1)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34437114
Proper citation: RRID:Addgene_177850 Copy
Species: Synthetic
Genetic Insert: KRaION2
Vector Backbone Description: Backbone Size:4005; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35427452
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177802 Copy
Species: Synthetic
Genetic Insert: mSandy2
Vector Backbone Description: Backbone Marker:Twist Bioscience; Backbone Size:5234; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35222925
Proper citation: RRID:Addgene_177760 Copy
Species: Homo sapiens
Genetic Insert: stonin2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11381094
Proper citation: RRID:Addgene_177885 Copy
Species: Escherichia coli
Genetic Insert: TcR (tetA/tetR)
Vector Backbone Description: Backbone Size:2677; Vector Backbone:pMK2010; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34801582
Comments: The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208).
Proper citation: RRID:Addgene_178086 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Nonviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178034 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Nonviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178032 Copy
Species: Homo sapiens
Genetic Insert: Toll-Interacting Protein
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPc3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32311122
Proper citation: RRID:Addgene_178050 Copy
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