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Species: Homo sapiens
Genetic Insert: E2-Crimson-NLS
Vector Backbone Description: Backbone Size:9075; Vector Backbone:CSII; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31599704
Comments: Please visit https://www.biorxiv.org/content/10.1101/587162v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_125263 Copy
Genetic Insert: 7D12 nanobody
Vector Backbone Description: Backbone Size:4282; Vector Backbone:pTrcHis; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30773887
Proper citation: RRID:Addgene_125268 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:8423; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30324134
Proper citation: RRID:Addgene_125256 Copy
Species: Homo sapiens
Genetic Insert: K-Ras 12V
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8668210
Comments: Constructed by Aylin Ulku at the Der lab. Insert: also a 3' BamHI site immediately adjacent to the 3' SalI site.
Proper citation: RRID:Addgene_12544 Copy
Genetic Insert: N MYC
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12540 Copy
Species: Mus musculus
Genetic Insert: MCK promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescriptKS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9844629
Comments: A 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK. A PolyA sequence was added downstream (see author's map, MCK-polyA-2). Please note that the restriction sites shown between the MCK promoter and polyA region in the author's map are NOT unique sites.
Proper citation: RRID:Addgene_12528 Copy
Species: Homo sapiens
Genetic Insert: human equilibrating nucleoside transporter 1
Vector Backbone Description: Backbone Marker:Keeney and Boeke; Backbone Size:5343; Vector Backbone:pJK148; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157888
Proper citation: RRID:Addgene_12536 Copy
Species: Bos taurus
Genetic Insert: bovine 70 kDa heat shock cognate protein full length wild type
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2950; Vector Backbone:pRSET_A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9346924
Proper citation: RRID:Addgene_12532 Copy
Species: Other
Genetic Insert: optimized RBS
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pFD116 with an optimized RBS to use in E. coli; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_125546 Copy
Vector Backbone Description: Backbone Marker:our group built; Backbone Size:10091; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression, in vitro expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31467278
Comments: Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_125547 Copy
Vector Backbone Description: Backbone Marker:we built; Backbone Size:8992; Vector Backbone:custom made; Vector Types:Mammalian Expression, Yeast Expression, in vitro expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31467278
Comments: Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_125548 Copy
Species: Homo sapiens
Genetic Insert: human Methylpurine DNA glycosylase
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5255; Vector Backbone:pIRESNeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16024643
Proper citation: RRID:Addgene_12548 Copy
Species: Mus musculus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12557 Copy
Species: Rattus norvegicus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12558 Copy
Species: Homo sapiens
Genetic Insert: AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
Vector Backbone Description: Backbone Size:15470; Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30970261
Proper citation: RRID:Addgene_125519 Copy
Species: Homo sapiens
Genetic Insert: EXPORTIN-5
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3900; Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11777942
Comments: For expression of this protein, supplement medium with2% ethanol; induce with 300 uM IPTG; add 25 mM beta-mercaptoethanol to the lysis buffer. Maintain 7 mM mercaptoethanol in all buffers for purification and storage.
Proper citation: RRID:Addgene_12553 Copy
Species: Homo sapiens
Genetic Insert: Exportin-5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pKmyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11777942
Proper citation: RRID:Addgene_12552 Copy
Species: Rattus norvegicus
Genetic Insert: CCAAT/Enhancer-binding Protein alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12578822
Proper citation: RRID:Addgene_12550 Copy
Species: Homo sapiens
Genetic Insert: genome-wide
Vector Backbone Description: Backbone Size:7566; Vector Backbone:pLCKO2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30970261
Proper citation: RRID:Addgene_125517 Copy
Species: Synthetic
Genetic Insert: po-roGFP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3961; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21372177
Proper citation: RRID:Addgene_125582 Copy
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