Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: Sec16L
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4731; Vector Backbone:pmGFP-c1 ( modified from pEGFP-c1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17192411
Comments: Please note that we have identified a K466R missense mutation in the Sec16L/Sec16A region of this plasmid, and the start codon may not have been correctly identified when constructing this plasmid which may have resulted in the gene being truncated. However, we do not think that this should impact plasmid functionality.
Proper citation: RRID:Addgene_15776 Copy
Species: Homo sapiens
Genetic Insert: Sec16S
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4731; Vector Backbone:pmGFPc1( modified from pEGFP-c1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17192411
Proper citation: RRID:Addgene_15775 Copy
Vector Backbone Description: Backbone Size:5401; Vector Backbone:pelB-MBP (DNASU access. no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: - Empty vector (kanamycin-R) adds TEV-protease-removable N-terminal PelB signal sequence + His10, plus potential C-term His6. Non-leaky expression due to T7lac promoter.
- Has an advantage in leaving no added amino acid residues following TEV protease cleavage of PelB signal sequence + His10. This advantage is made possible by cloning into the two BseRI restriction sites.
- Subcloning requires ligation-independent cloning and use of the two BseRI restriction sites that are present in this empty vector. The two BseRI sites span a removable 432 nucleotide sequence. Cloning into the BseRI-digested vector can be achieved using a ligation-independent system that is dependent on homologous recombination, such as the Takara In-Fusion HD Cloning Plus system, which requires simply designing the PCR-derived insert (containing the protein of interest) to contain 15 bp at each end that matches the 15 bp at each end of the linearized parent vector. In other words, add these 15 bp, AACCTGTACTTCCAA, to the 5' end of the PCR FORWARD primer, and add these 15 bp, GTGGTGGTGCTCGAG, to the 5' end of the PCR REVERSE primer.
- The sequence of the added N-terminus is the PelB signal sequence (M1-A22 of GenBank #AAA24848) + 13 aa linker + His10-tag + 4 aa linker + TEV protease cleavage site. The N-terminal protein sequence is: MKYLLPTAAAGLLLLAAQPAMA + MDIGINSDPNSSS + HHHHHHHHHH + PMGS + ENLYFQ*X, where * is the TEV protease cleavage site and X is the N-terminal residue of the introduced protein of interest. Note that TEV protease cleaves most efficiently when X = S, G, A, M; for other compatible residues see Kapust et al 2002, PMID 12074568.
- A C-terminal His6-tag is possible: if no stop codon is included in the cloned insert, the sequence LEHHHHHH is added to the C-terminus.
- The PelB signal sequence directs the protein of interest to the E. coli inner membrane. Whether the protein traverses the inner membrane is dependent on the protein of interest.
- BseRI cuts 8-10 nucleotides outside of its own recognition sequence and is a non-palindromic site. The placement & orientation of the two BseRI sites eliminates all of the BseRI recognition sequence in the resulting subclone.
- To verify the PCR-generated insert sequence, use sequencing primers T7 (TAATACGACTCACTATAGGG), T7-Ter (GCTAGTTATTGCTCAGCGG).
Proper citation: RRID:Addgene_157741 Copy
Species: Homo sapiens
Genetic Insert: AURKA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30070631
Proper citation: RRID:Addgene_157755 Copy
Species: Homo sapiens
Genetic Insert: TOMM22
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30070631
Proper citation: RRID:Addgene_157762 Copy
Species: Homo sapiens
Genetic Insert: MFF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30070631
Proper citation: RRID:Addgene_157760 Copy
Species: Mus musculus
Genetic Insert: H4
Vector Backbone Description: Backbone Size:4750; Vector Backbone:Dendra2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32783931
Proper citation: RRID:Addgene_157815 Copy
Species: Mus musculus
Genetic Insert: H3
Vector Backbone Description: Backbone Size:4750; Vector Backbone:Dendra2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32783931
Proper citation: RRID:Addgene_157814 Copy
Species: Mus musculus
Genetic Insert: H2A
Vector Backbone Description: Backbone Size:4750; Vector Backbone:Dendra2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32783931
Proper citation: RRID:Addgene_157816 Copy
Species: SARS-CoV-2
Genetic Insert: N
Vector Backbone Description: Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33200826
Proper citation: RRID:Addgene_157869 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32870271
Proper citation: RRID:Addgene_157866 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32870271
Proper citation: RRID:Addgene_157865 Copy
Species: SARS-CoV-2
Genetic Insert: N
Vector Backbone Description: Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33200826
Comments: Please note: This plasmid contains a D15H mutation in MBP. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_157872 Copy
Species: SARS-CoV-2
Genetic Insert: N
Vector Backbone Description: Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33200826
Proper citation: RRID:Addgene_157871 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 56399 was replaced with mGold.
"C-18" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that older versions of SnapGene may annotate the flurophore as "mVenus;" we have confirmed that this plasmid encodes mGold.
Proper citation: RRID:Addgene_158008 Copy
Species: Homo sapiens
Genetic Insert: Calnexin
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 56324 was replaced with mGold.
"N-14" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158004 Copy
Species: Mus musculus
Genetic Insert: MannII
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 56332 was replaced with mGold.
"N-10" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158005 Copy
Species: Mus musculus
Genetic Insert: CAF1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 55953 was replaced with mGold.
"C-10" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158003 Copy
Species: Synthetic
Genetic Insert: de novo designed protein RO2_10
Vector Backbone Description: Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32855341
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.14.041772v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158019 Copy
Species: Synthetic
Genetic Insert: de novo designed protein RO2_9
Vector Backbone Description: Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32855341
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.14.041772v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158018 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
