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Species: Saccharomyces cerevisiae
Genetic Insert: pREV1-mNeonGreen-tENO2
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233834 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NLS-VP48-2XOaf1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38079574
Comments: This part lacks a stop codon and is designed to be used with the ADH1 terminator, which has an early upstream stop codon
Proper citation: RRID:Addgene_233828 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NLS-VP48
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233824 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NLS-VP48-2XOaf1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233826 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP48
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233827 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LexA-NES
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233822 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233823 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5H28
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42555168
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)
Proper citation: RRID:Addgene_234184 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3-deltaPEST
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: Amino acid 413 was mutated to a stop codon using site-directed mutagenesis, creating a truncation mutant of Ste3s C-tail at this residue in the pRS415-STE3pr-MFA-Igkappa-FAP-STE3.
Proper citation: RRID:Addgene_221118 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ANP1-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of ANP1 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ARB1 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232888 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prp8
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CEN6-ARS4-HIS3
Vector Backbone Description: Vector Backbone:pXTK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40080684
Proper citation: RRID:Addgene_229358 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Swap1
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231879 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 solvvol
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231877 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 9acidA
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231867 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 SATtoG KtoR
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231861 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 S/ArotoL
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231880 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 WW+
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Aro3+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232990 Copy
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