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Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129016 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129011 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129012 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129013 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129019 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129010 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129005 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129006 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129001 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35NM
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:8600; Vector Backbone:pGAN147; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: This is the basic yTRAP sensor to sense aggregation of Sup35 or [PSI+]. It should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.
Proper citation: RRID:Addgene_129203 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNQ1
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:8600; Vector Backbone:pGAN147; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: This is the basic yTRAP sensor to sense aggregation of Rnq1 or [RNQ+]. It should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.
Proper citation: RRID:Addgene_129204 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FlpO-2A-Cre
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pOG44; Vector Types:Mammalian Expression, Cre/Lox, Unspecified, Episomal expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31539496
Proper citation: RRID:Addgene_129419 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 15xUAS-∆pes-10-Flp-SL2
Vector Backbone Description: Vector Backbone:pBN412; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32550473
Comments: 15xUAS sequence from pHW394 inserted into pBN412
Proper citation: RRID:Addgene_129555 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADP-ribosylation factor GTPase-activating protein
Vector Backbone Description: Vector Backbone:PQE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129484 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Glo3 ΔGRM ΔC
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc20/AID(71-114)-tCYC1-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31606371
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast.
The work is currently under review.
Proper citation: RRID:Addgene_130269 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 F130W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L553W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1306 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y507W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1305 Copy
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