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Species: Other
Genetic Insert: Portal
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188824 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-IKZF3-NTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185774 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-IKZF3-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185779 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-AID-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185778 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-HALO-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185777 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-SMASH-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185776 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:SFFV-IKZF3-NTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185764 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:SFFV-AID-NTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185763 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:SFFV-HALO-NTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185762 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:SFFV-DTAG-NTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185760 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:SFFV-IKZF5-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185769 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:SFFV-HALO-CTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185767 Copy
Species: Other
Genetic Insert: ecTadA(8e)-nSpRY
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36076084
Proper citation: RRID:Addgene_185671 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Marker:Näätsaari et al., 2012; Vector Backbone:pPpT4; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36043049
Proper citation: RRID:Addgene_189724 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:PGK-DTAG-NTERM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36127368
Proper citation: RRID:Addgene_185775 Copy
Species: other
Genetic Insert: gRNA, Cas9, firefly luciferase, bar
Vector Backbone Description: Vector Backbone:pTRANS230d; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35689624
Comments: IS4 family transposase (genbank ID:WP_006250222.1) does not affect plasmid function.
Proper citation: RRID:Addgene_190246 Copy
Species: other
Genetic Insert: gRNA, Cas9, firefly luciferase, bar
Vector Backbone Description: Vector Backbone:pTRANS230d; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35689624
Comments: IS4 family transposase (genbank ID:WP_006250222.1) does not affect plasmid function.
Proper citation: RRID:Addgene_190247 Copy
Species: Other
Genetic Insert: Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
Vector Backbone Description: Backbone Size:2828; Vector Backbone:pHH21; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35694292
Proper citation: RRID:Addgene_187924 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34124437
Comments: pSiMPls_N should be co-transformed with pSiMPls_C (or vice versa) in E. coli to get a reconstituted functional aminoglycoside adenylyltransferase thereby conferring resistance towards spectinomycin/streptomycin. The plasmids possess no additional antibiotic resistance gene.
Please see the Sequences link for the individual plasmid sequences.
An E. coli bacterial stab from Addgene will have both the plasmids pSiMPls_N + pSiMPls_C. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Proper citation: RRID:Addgene_167203 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9173; Vector Backbone:pLVX-TetOne-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34352222
Comments: pLVX-TetOne-Puro backbone from pLVX-TetOne-Puro-hAXL (Plasmid #124797)
EGFP insert from Lenti-Cas9-gRNA-GFP (Addgene #124770)
Proper citation: RRID:Addgene_171123 Copy
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