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Species: Homo sapiens
Genetic Insert: human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945)
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5569; Vector Backbone:pSBtet-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538252
Proper citation: RRID:Addgene_119189 Copy
Species: Homo sapiens
Genetic Insert: human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945)
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5569; Vector Backbone:pSBtet-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538252
Proper citation: RRID:Addgene_119190 Copy
Species: Homo sapiens
Genetic Insert: human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP]
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5569; Vector Backbone:pSBtet-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538252
Proper citation: RRID:Addgene_119187 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 2 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119205 Copy
Species: Homo sapiens
Genetic Insert: sh1miR targeting NF1
Vector Backbone Description: Backbone Marker:Modified from pGIPZ; Backbone Size:9359; Vector Backbone:pLV-H1-SGIPZ; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:30408279
Comments: The miRNA may also target additional species.
The vector also expresses turboGFP.
The restriction sites useful for verifying the vector and distinguishing from the empty vector (Addgene #119217) are indicated on the map; we suggest using MluI+SalI or MluI+BsrGI. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119206 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 2 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119203 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 1 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119204 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 1 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119200 Copy
Species: Homo sapiens
Genetic Insert: NKX2-1
Vector Backbone Description: Vector Backbone:pMSCV-hygromycin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17925434
Proper citation: RRID:Addgene_119173 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I (C21Y)
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119304 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I (G20V)
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119308 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I (R39H)
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119309 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11929 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: Venus was developed by Miyawaki and colleagues. The reference is Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90. The EYFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11931 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11930 Copy
Species: Homo sapiens
Genetic Insert: KIAA1524
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28174209
Comments: 522 AGA-> GAT mutation
Proper citation: RRID:Addgene_119289 Copy
Species: Homo sapiens
Genetic Insert: KIAA1524
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28174209
Proper citation: RRID:Addgene_119287 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 or pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Addgene Note: Please see the Addgene sequencing result to view the fluorophore sequence.
Proper citation: RRID:Addgene_11937 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11936 Copy
Species: Homo sapiens
Genetic Insert: PPME1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26499835
Proper citation: RRID:Addgene_119292 Copy
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