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Species: Homo sapiens
Genetic Insert: TERRA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR/D-hH1; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27537914
Comments: Depositing Scientist Sequence may not contain all correct information about the telomere sequence repeats such as canonical and variant repeat positions, and precise information about insert length. Further, the Addgene Partial NGS Result does not include the entire region of telomere repeats. Note that the BamHI site was destroyed during cloning.
Proper citation: RRID:Addgene_111383 Copy
Species: Other
Genetic Insert: translational initiation factor 3
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111484 Copy
Species: Other
Genetic Insert: Arg-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111481 Copy
Species: Other
Genetic Insert: Cys-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111482 Copy
Species: Other
Genetic Insert: translational elongation factor 4
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111472 Copy
Species: Other
Genetic Insert: Lys-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111470 Copy
Species: Other
Genetic Insert: Asp-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111476 Copy
Species: Other
Genetic Insert: ribosome recycling factor
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111479 Copy
Species: Other
Genetic Insert: Val-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111463 Copy
Species: Other
Genetic Insert: Thr-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111469 Copy
Species: Homo sapiens
Genetic Insert: potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111451 Copy
Species: Homo sapiens
Genetic Insert: Sox9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27566146
Comments: Reference for original WT SOX9 plasmid - https://www.ncbi.nlm.nih.gov/pubmed/?term=20651055
We used these primers for SDM Sox9 F7site5mut1F: 5'-ggcccaccggccccacccAccAcccccaaaa-3' Sox9 F7site5mut1R: 5'-ttttgggggTggTgggtggggccggtgggcc-3'
Proper citation: RRID:Addgene_111455 Copy
Species: Homo sapiens
Genetic Insert: potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111398 Copy
Species: Synthetic
Genetic Insert: GCaMP6m-XC
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29666364
Proper citation: RRID:Addgene_111543 Copy
Species: Other
Genetic Insert: Met-tRNA formyltransferase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111492 Copy
Species: Xenopus laevis
Genetic Insert: calmodulin [Xenopus laevis]
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111499 Copy
Species: Homo sapiens
Genetic Insert: human C73M-Kv2.1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:peGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_111535 Copy
Species: Homo sapiens
Genetic Insert: human C73D-Kv2.1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_111534 Copy
Species: Homo sapiens
Genetic Insert: human C73W-Kv2.1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:peGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_111537 Copy
Species: Homo sapiens
Genetic Insert: human Kv2.1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:peGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_111538 Copy
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