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Species: Other
Genetic Insert: pTet-LbuCas13a + crRNA negative control
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36316451
Proper citation: RRID:Addgene_186240 Copy
Species: Other
Genetic Insert: BBR1 backbone + T4 phage homology
Vector Backbone Description: Vector Backbone:BBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36316451
Proper citation: RRID:Addgene_186247 Copy
Species: Other
Genetic Insert: pTet-RfxCas13d + crRNA negative control
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36316451
Proper citation: RRID:Addgene_186241 Copy
Species: Other
Genetic Insert: pTet-eLbuCas13a + crRNA negative control
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36316451
Proper citation: RRID:Addgene_186239 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-Flag-UTR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36202974
Proper citation: RRID:Addgene_183236 Copy
Species: Other
Genetic Insert: rAPOBEC1-neNme2-C-2xUGI
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36076084
Proper citation: RRID:Addgene_183679 Copy
Species: Other
Genetic Insert: 2xtTA
Vector Backbone Description: Backbone Size:4805; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35545715
Proper citation: RRID:Addgene_191205 Copy
Species: Other
Genetic Insert: 3X GFP
Vector Backbone Description: Backbone Size:5032; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31495573
Proper citation: RRID:Addgene_191204 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Addgene #25890; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of Luciferase with C-terminal V5 tag.
Proper citation: RRID:Addgene_98580 Copy
Species: Other
Genetic Insert: MS2
Vector Backbone Description: Backbone Size:3100; Vector Backbone:Derived from pDMSD-Y2 (Kostecki et al., 2010, 10.1371/journal.pone.0009225); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25080893
Proper citation: RRID:Addgene_98587 Copy
Species: Other
Genetic Insert: shLacZ
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the TRC (TRCN0000072238). Used as control knockdown vector.
Proper citation: RRID:Addgene_98632 Copy
Species: Other
Genetic Insert: mEos3.2-A69T
Vector Backbone Description: Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Proper citation: RRID:Addgene_98571 Copy
Species: Other
Genetic Insert: mKikGR-V69T
Vector Backbone Description: Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Proper citation: RRID:Addgene_98574 Copy
Species: Other
Genetic Insert: pcDronpa2-A69T
Vector Backbone Description: Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Proper citation: RRID:Addgene_98578 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374 (Addgene 59896); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98698 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Addgene #25896; Backbone Size:9377; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of LacZ with C-terminal V5 tag.
Proper citation: RRID:Addgene_98579 Copy
Species: Other
Genetic Insert: NS1
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28323816
Proper citation: RRID:Addgene_99022 Copy
Species: Other
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99134 Copy
Species: Other
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99113 Copy
Species: Other
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99114 Copy
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