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Species: Homo sapiens
Genetic Insert: HRas 12V 37G
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12589 Copy
Species: Homo sapiens
Genetic Insert: N-term truncated mut of Raf-1
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12593 Copy
Species: Bos taurus
Genetic Insert: p110-CAAX
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBabe hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Comments: Myc tagged p110-CAAX was cut out of pBabe-puro p110-CAAX using BamHI and ligated into BamHI cut pBabe-hygro.
Proper citation: RRID:Addgene_12591 Copy
Species: Homo sapiens
Genetic Insert: HRas 12V 40C
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12590 Copy
Species: Homo sapiens
Genetic Insert: RhoA Biosensor
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pBabe-Puro-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547516
Comments: Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Proper citation: RRID:Addgene_12602 Copy
Species: Homo sapiens
Genetic Insert: EGFP-Cdc42 (Q61L)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15361624
Comments: Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Proper citation: RRID:Addgene_12600 Copy
Species: Sulfolobus solfataricus
Genetic Insert: IS200 transposase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET-21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16340015
Proper citation: RRID:Addgene_12604 Copy
Vector Backbone Description: Backbone Marker:Kabin Xie; Vector Backbone:pRGEB32; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33917747
Comments: This vector has been redesigned for use in Zea mays. Published protocols for using pRGEB32 should be used for this vector.
Proper citation: RRID:Addgene_126072 Copy
Genetic Insert: dCas9 fusion with KRAB
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:piggyBac cargo vector; Vector Types:Mammalian Expression, CRISPR, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31101683
Comments: Please visit https://www.biorxiv.org/content/10.1101/448803v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126030 Copy
Species: Synthetic
Genetic Insert: rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:piggyBac cargo vector; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224646
Proper citation: RRID:Addgene_126034 Copy
Genetic Insert: humanized S. pyrogenes Cas9
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:piggyBac cargo vector; Vector Types:Mammalian Expression, CRISPR, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31101683
Comments: Please visit https://www.biorxiv.org/content/10.1101/448803v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126029 Copy
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:8660; Vector Backbone:piggyBac cargo vector; Vector Types:Mammalian Expression, CRISPR, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31101683
Comments: Please visit https://www.biorxiv.org/content/10.1101/448803v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126028 Copy
Genetic Insert: His6-Sumo-CfaC-MBP
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126018 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31285614
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/04/434720.full.pdf for BioRxiv preprint
Proper citation: RRID:Addgene_126005 Copy
Genetic Insert: PSD95.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:5603; Vector Backbone:AAV2-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32674057
Proper citation: RRID:Addgene_126216 Copy
Genetic Insert: Gephyrin.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:5603; Vector Backbone:AAV2-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32674057
Proper citation: RRID:Addgene_126217 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126201 Copy
Genetic Insert: Green Glifon50
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30869867
Proper citation: RRID:Addgene_126206 Copy
Genetic Insert: Green Glifon600
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30869867
Proper citation: RRID:Addgene_126207 Copy
Genetic Insert: Green Glifon4000
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30869867
Proper citation: RRID:Addgene_126208 Copy
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