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Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Comments: PAGFP is a photoactivatable version of GFP. It is essentially the same as the standard EGFP-C1 from clontech, but contains the mutations L64F/T65S/V163A/T203H, and conatins an A206K mutation to disrupt dimerization.
In total, the PAGFP-Ubiquitin insert is 983bp.
Proper citation: RRID:Addgene_11933 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin KO.G76V
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Proper citation: RRID:Addgene_11932 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16702407
Proper citation: RRID:Addgene_11935 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin KO
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16702407
Proper citation: RRID:Addgene_11934 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119315 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119316 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119313 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119311 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119312 Copy
Species: Homo sapiens
Genetic Insert: E6-AP III
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119318 Copy
Species: Homo sapiens
Genetic Insert: Stratifin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1 His C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15731107
Proper citation: RRID:Addgene_11947 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK?; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428519
Comments: Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
Proper citation: RRID:Addgene_11944 Copy
Species: Homo sapiens
Genetic Insert: NEMO
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16547522
Proper citation: RRID:Addgene_11966 Copy
Species: Homo sapiens
Genetic Insert: NEMOL329P
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547522
Proper citation: RRID:Addgene_11967 Copy
Species: Homo sapiens
Genetic Insert: ATF6
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pCMV-3xFLAG-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11821395
Comments: See author's map for cloning details.
Proper citation: RRID:Addgene_11975 Copy
Species: Homo sapiens
Genetic Insert: NEMOL329P
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCMVTAG2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16547522
Comments: Please note that Addgene's quality control sequence confirmed 2 additional amino acids changes in the NEMO ORF - A211T and L301M.
Proper citation: RRID:Addgene_11971 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8336707
Comments: See author's map and paper for cloning details. pCGN: expression is driven by CMV promoter
Proper citation: RRID:Addgene_11977 Copy
Species: Homo sapiens
Genetic Insert: MKL1
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pCMV-3xFLAG-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12944485
Proper citation: RRID:Addgene_11978 Copy
Species: Homo sapiens
Genetic Insert: PSAM4 GlyR IRES eGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30872534
Proper citation: RRID:Addgene_119744 Copy
Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679426
Proper citation: RRID:Addgene_119694 Copy
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