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Species: Synthetic
Genetic Insert: AOX
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: (Gene Synthesis by GeneArt)
Proper citation: RRID:Addgene_111661 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033366
Proper citation: RRID:Addgene_111595 Copy
Species: Mus musculus
Genetic Insert: mU6-sgRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033366
Comments: Addgene NGS results identified a V163A variant in EGFP, which does not affect plasmid function.
Proper citation: RRID:Addgene_111596 Copy
Species: Mus musculus
Genetic Insert: H2Kb
Vector Backbone Description: Backbone Marker:Addgene # 60523; Backbone Size:5661; Vector Backbone:pSBbi-pur; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111623 Copy
Genetic Insert: Venus-LEQLE-SIINFEKL-TEW
Vector Backbone Description: Backbone Marker:Addgene # 26553; Vector Backbone:pT2/SV-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111624 Copy
Genetic Insert: yellow pre-eGRASP(p30)
Vector Backbone Description: Backbone Size:4375; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111588 Copy
Genetic Insert: cyan pre-eGRASP(p32)
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700265
Proper citation: RRID:Addgene_111589 Copy
Species: Mus musculus
Genetic Insert: TOMM20
Vector Backbone Description: Backbone Marker:Mark Van Delft; Backbone Size:6969; Vector Backbone:pMIH/MSCV-IRES-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29472455
Proper citation: RRID:Addgene_111626 Copy
Species: Homo sapiens
Genetic Insert: PDK1
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11062_G08)
Proper citation: RRID:Addgene_111672 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for IPTG-inducible expression of APEX2 in the E. coli cytoplasm. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111702 Copy
Species: Mus musculus
Genetic Insert: Mef2c, Tbx5 and Gata4
Vector Backbone Description: Backbone Size:5871; Vector Backbone:pMx; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25416133
Proper citation: RRID:Addgene_111810 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEMT-easy; Vector Types:cloning intermediate; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28526819
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111812 Copy
Species: Homo sapiens
Genetic Insert: GGA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11177 Copy
Species: Homo sapiens
Genetic Insert: RBAP2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11174 Copy
Species: Homo sapiens
Genetic Insert: BLM
Vector Backbone Description: Backbone Marker:Invitrogen (thermoFisher); Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971187
Proper citation: RRID:Addgene_111766 Copy
Species: Mus musculus
Genetic Insert: Mef2c, Tbx5 and Gata4
Vector Backbone Description: Backbone Marker:Cell biolabs; Backbone Size:5900; Vector Backbone:pMx-puromycin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25416133
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111809 Copy
Genetic Insert: EYFP from Aequorea victoria
Vector Backbone Description: Backbone Marker:Available at Addgene (#11160); Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14603031
Proper citation: RRID:Addgene_11180 Copy
Species: Synthetic
Genetic Insert: 2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
Vector Backbone Description: Vector Backbone:GW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30315152
Proper citation: RRID:Addgene_111759 Copy
Species: Synthetic
Genetic Insert: 2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
Vector Backbone Description: Vector Backbone:β-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30315152
Proper citation: RRID:Addgene_111758 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111741 Copy
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