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Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 DBD
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232985 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 WTSLFD+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 StoL
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232981 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Aro2+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232982 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Aro3+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232983 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 solvvol W+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 S/ArotoL
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232979 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R++
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232973 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 F108A
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R++ W+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Proline
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 L113A
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FUN30, CUE domain mutated
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8964; Vector Backbone:pYES2.1/V5-His/lacZ; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956593
Proper citation: RRID:Addgene_46792 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FUN30
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8964; Vector Backbone:pYES2.1/V5-His/lacZ; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956593
Proper citation: RRID:Addgene_46790 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FUN30 without CUE domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8964; Vector Backbone:pYES2.1/V5-His/lacZ; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956593
Proper citation: RRID:Addgene_46791 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FUN30
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8964; Vector Backbone:pYES2.1/V5-His/lacZ; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956593
Proper citation: RRID:Addgene_46789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sgTEF1 promoter
Vector Backbone Description: Backbone Size:5083; Vector Backbone:AH057; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23849981
Comments: gRNA target sequence TTGATATTTAAGTTAATAAA
Proper citation: RRID:Addgene_46922 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pBbA5c; Vector Types:Bacterial Expression, Synthetic Biology, BglBrick; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23727191
Comments: The R364S mutation in the MK is not known to alter the function. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_47048 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2961; Vector Backbone:pPCR-Script Amp SK(+); Vector Types:PCR template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24639370
Comments: Primer sequences (5' to 3'): U2, CGTACGCTGCAGGTCGAC; D2, ATCGATGAATTCGAGCTCG.
Proper citation: RRID:Addgene_47554 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kar2-SS
Vector Backbone Description: Backbone Marker:John Dueber Lab, https://doi.org/10.1021/sb500366v; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37009624
Proper citation: RRID:Addgene_200132 Copy
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