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Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111745 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111742 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111730 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the periplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111699 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111729 Copy
Species: Gallus gallus
Genetic Insert: VinculinTS (I997A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29642037
Proper citation: RRID:Addgene_111828 Copy
Species: Homo sapiens
Genetic Insert: Ple320
Vector Backbone Description: Backbone Marker:Elizabeth Simpson (Addgene plasmid # 111895); Backbone Size:4999; Vector Backbone:pEMS2131; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. Please note - an 11 bp insertion, which introduces a StuI site upstream of 5' ITR, was found during Addgene's Quality Control process.
Proper citation: RRID:Addgene_111896 Copy
Species: Homo sapiens
Genetic Insert: Interleukin 6
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30016456
Proper citation: RRID:Addgene_111933 Copy
Species: Homo sapiens
Genetic Insert: Signal transducer and activator of transcription 3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30016456
Proper citation: RRID:Addgene_111934 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pUC; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29785042
Proper citation: RRID:Addgene_111819 Copy
Vector Backbone Description: Backbone Size:6997; Vector Backbone:LentiV_puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_111886 Copy
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111873 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111874 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111878 Copy
Genetic Insert: yFusionRed
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6902; Vector Backbone:P415 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631438
Proper citation: RRID:Addgene_111916 Copy
Species: Mus musculus
Genetic Insert: BICDN(1-400)-GFP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29420470
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111862 Copy
Species: Homo sapiens
Genetic Insert: His-ZZ-LTLT-SNAPf-DYNC1H1
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:3063; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Gentamicin
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
This plasmid replaces pDyn3 from Schlager et al. 2014.
Proper citation: RRID:Addgene_111903 Copy
Species: Homo sapiens
Genetic Insert: Ple345
Vector Backbone Description: Backbone Marker:Elizabeth Simpson (Addgene plasmid # 111895); Backbone Size:4999; Vector Backbone:pEMS2131; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. Please note - an 11 bp insertion, which introduces a StuI site upstream of 5' ITR, was found during Addgene's Quality Control process.
Proper citation: RRID:Addgene_111901 Copy
Species: Homo sapiens
Genetic Insert: RNASEH1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:ppyCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29104020
Comments: Due to high GC content of the CAG promoter, there are some discrepancies in the promoter sequence. These are not known to affect the plasmid function.
Proper citation: RRID:Addgene_111906 Copy
Species: Homo sapiens
Genetic Insert: RNASEH1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:ppyCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29104020
Comments: Due to high GC content of the CAG promoter, there are some discrepancies in the promoter sequence. These are not known to affect the plasmid function.
Proper citation: RRID:Addgene_111905 Copy
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