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Species: Homo sapiens
Genetic Insert: Smad2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9732876
Comments: See author's map for details.
Proper citation: RRID:Addgene_12623 Copy
Species: Homo sapiens
Genetic Insert: Smad1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9732876
Comments: See author's map for details.
Proper citation: RRID:Addgene_12622 Copy
Species: Broad range of bacteria
Genetic Insert: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
Vector Backbone Description: Backbone Size:7846; Vector Backbone:RSF1010Y25F_aph1_PconII-GFPmut2; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25074377
Proper citation: RRID:Addgene_120080 Copy
Species: N. meningitidis
Genetic Insert: dNmeCas9
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30518407
Proper citation: RRID:Addgene_120077 Copy
Species: Homo sapiens
Genetic Insert: mScarlet-i
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120054 Copy
Species: Homo sapiens
Genetic Insert: N19 3'UTR
Vector Backbone Description: Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16308420
Comments: NID1 3'UTR in renilla luciferase reporter plasmid.
Proper citation: RRID:Addgene_12016 Copy
Species: Mus musculus
Genetic Insert: kinesin family member 5A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31100061
Proper citation: RRID:Addgene_120163 Copy
Species: Mus musculus
Genetic Insert: kinesin family member 1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31100061
Proper citation: RRID:Addgene_120165 Copy
Species: Mus musculus
Genetic Insert: BICD cargo adaptor 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31100061
Proper citation: RRID:Addgene_120168 Copy
Species: Homo sapiens
Genetic Insert: mAID-Clover
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120037 Copy
Species: Homo sapiens
Genetic Insert: 3xHA
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120039 Copy
Species: Homo sapiens
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120024 Copy
Genetic Insert: g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
Vector Backbone Description: Vector Backbone:pEG302; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30760722
Proper citation: RRID:Addgene_120249 Copy
Species: Mus musculus
Genetic Insert: Lem2
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31142202
Proper citation: RRID:Addgene_120245 Copy
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31142202
Comments: For LIC cloning: cut with SrfI before T4 polymerase treatment
Proper citation: RRID:Addgene_120247 Copy
Species: Other
Genetic Insert: intB13 integrase casette
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120230 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120231 Copy
Genetic Insert: cre
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAAV; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30886408
Proper citation: RRID:Addgene_120219 Copy
Species: Other
Genetic Insert: sfGFP/mScarletP2A-mcs
Vector Backbone Description: Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that sfGFP can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A
The associated publication utilized mNeon instead of sfGFP/mClover3, but mNeon has is exceedingly difficult to share. Therefore, these two plasmids (120261 and 120262) are made available, using sfGFP and mClover3 instead of mNeon. These plasmids have been tested, and are equivalent to the mNeon variant.
Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pDYSCKO cassette-ADE1 targeting guide
Vector Backbone Description: Backbone Size:6467; Vector Backbone:pDYSCKO; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30097473
Comments: See Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast. (PMID: 30097473) for detailed protocols for gRNA cloning and mutagenesis protocol.
Proper citation: RRID:Addgene_120264 Copy
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