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Species: Saccharomyces cerevisiae
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133591 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: p1k-SNF7-GFP
Vector Backbone Description: Vector Backbone:KT209; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31481383
Proper citation: RRID:Addgene_133671 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTPI1-mTagBFP2-SKL
Vector Backbone Description: Vector Backbone:Clhn-mTagBFP2-Trp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31481383
Proper citation: RRID:Addgene_133670 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: p1k-COX4-mTagBFP2
Vector Backbone Description: Vector Backbone:Clhn-mTagBFP2-Trp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31481383
Proper citation: RRID:Addgene_133668 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Backbone Marker:J. Clos and Brandau, 1994; Backbone Size:3000; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9182769
Proper citation: RRID:Addgene_1092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Backbone Marker:J. Clos and Brandau, 1994; Backbone Size:3000; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9182769
Proper citation: RRID:Addgene_1095 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rpb1-C67Y
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS415 (with modifications); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16510790
Proper citation: RRID:Addgene_10982 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPB1
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS415 (with modifications); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16510790
Proper citation: RRID:Addgene_10981 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rpb1-D261N
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS415 (with modifications); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16510790
Proper citation: RRID:Addgene_10986 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rpb1-N488D
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS415 (with modifications); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16510790
Proper citation: RRID:Addgene_10988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPT6
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10993 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rpb1-E1230K
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS415 (with modifications); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16510790
Proper citation: RRID:Addgene_10990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BUD32
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hUD recombination system
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result.
Proper citation: RRID:Addgene_11005 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11004 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hUI recombination system
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result.
Proper citation: RRID:Addgene_11006 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSM1
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSM1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11002 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pgal1TRP1URA3
Vector Backbone Description: Backbone Size:3786; Vector Backbone:pYC6-CT/HIS3 hybrid; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.
Proper citation: RRID:Addgene_11009 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990037
Proper citation: RRID:Addgene_1105 Copy
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