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Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV-N-Flag-HA-IRES-PURO; Vector Types:Mammalian Expression, Retroviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_41033 Copy
Vector Backbone Description: Backbone Size:4146; Vector Backbone:pTKIP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20047970
Proper citation: RRID:Addgene_41065 Copy
Vector Backbone Description: Backbone Size:10065; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid for constitutive lentiviral expression.
There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence.
Alternate plasmid names:
pLX307,
SV40-puro; EF1a-gateway-V5 tag
Proper citation: RRID:Addgene_41392 Copy
Vector Backbone Description: Backbone Size:9308; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid for constitutive lentiviral expression.
There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence.
Alternate plasmid names:
pLX306,
SV40-puro; PGK-gateway-V5 tag
Proper citation: RRID:Addgene_41391 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 41393; Backbone Size:9396; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid expresses puro-2A-rtTA-VP16 from the PGK promoter, so it can be used as an 'all-in-one' dox on system.
Alternate plasid name:
TRE-gateway-V5; PGK-puro-2A-rtTA
Proper citation: RRID:Addgene_41395 Copy
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid# 14140); Backbone Size:6972; Vector Backbone:pAG306GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23172144
Comments: This plasmid was generated by ligation of a SmaI-digested fusion PCR product that contained two ~500-base-pair regions of chromosome 1 flanking a NotI site into AatII-digested pAG306-GPD-ccdB.
Proper citation: RRID:Addgene_41894 Copy
Genetic Insert: TAP Tag::mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42161 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42152 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 19780; Backbone Size:9600; Vector Backbone:FUdeltaGW-rtTA; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22174877
Comments: The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway.
Proper citation: RRID:Addgene_43915 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI
Vector Backbone Description: Vector Backbone:pAM1667; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NS2.1 vector
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942
500bp PvuII-SalI promoter fragment of psbAI inserted into SalI-StuI digested pAM1667 (luxCDE).
Proper citation: RRID:Addgene_40242 Copy
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:0; Vector Backbone:N/A; Vector Types:N/A; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22957093
Proper citation: RRID:Addgene_40802 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 31829; Vector Backbone:pminiTol2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid contains Tol2 transposon.
Proper citation: RRID:Addgene_40970 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17086198
Proper citation: RRID:Addgene_37843 Copy
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pKD4; Vector Types:E.coli genomic integration; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23799955
Comments: This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between arsB homologous arms and integrated at the arsB locus.
This plasmid has been found exist in our stock as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_46763 Copy
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pKD4; Vector Types:E.coli genomic integration; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23799955
Comments: This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between lacZ homologous arms and integrated at the lacZ locus.
Proper citation: RRID:Addgene_46764 Copy
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pKD4; Vector Types:E.coli genomic integration; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23799955
Comments: This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between rbsAR homologous arms and integrated at the rbsAR locus.
Proper citation: RRID:Addgene_46765 Copy
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:4874; Vector Backbone:pHAGE-CAGS-W; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21307310
Proper citation: RRID:Addgene_46948 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5824; Vector Backbone:pBADnLIC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47075 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47073 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5519; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47079 Copy
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