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Species: Saccharomyces cerevisiae
Genetic Insert: SET1
Vector Backbone Description: Backbone Size:6442; Vector Backbone:pGLx2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22851644
Proper citation: RRID:Addgene_53257 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dbp9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63743 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Drs1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63744 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pAG414GPD; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 contains D839A and N863A mutations relative to reference sequence (WP_010922251.1). Depositor states that these mutations do not affect function.
Proper citation: RRID:Addgene_63801 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nsr1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63769 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nog2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63764 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nop2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63766 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mak16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63762 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tma16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63779 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rrp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63773 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Puf6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Comments: The Lifeact sequence was initially reported in Riedl, J. et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods 5, 605–607 (2008).
Please cite the following article as follows:
Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
Proper citation: RRID:Addgene_64048 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: adenine phosphoribosyltransferase
Vector Backbone Description: Backbone Marker:Qiagen; Vector Backbone:pQE32; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11535055
Proper citation: RRID:Addgene_64028 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast-optimized dCas9
Vector Backbone Description: Vector Backbone:pRS304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64279 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter
Vector Backbone Description: Backbone Marker:Mateus and Avery 2000; Vector Backbone:GFPCLN2PEST-pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25886317
Comments:
Proper citation: RRID:Addgene_64406 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUC2 promoter
Vector Backbone Description: Backbone Marker:Mateus and Avery 2000; Vector Backbone:GFPCLN2PEST-pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25886317
Comments:
Proper citation: RRID:Addgene_64405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: g1 gRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced GFP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64389 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: c7 gRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64385 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced BFP2
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64390 Copy
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