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Vector Backbone Description: Backbone Size:9506; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35250 Copy
Vector Backbone Description: Backbone Size:7773; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35253 Copy
Vector Backbone Description: Backbone Size:9298; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35247 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33577114
Proper citation: RRID:Addgene_167300 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167694 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167686 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUBQ4-2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167685 Copy
Species: Staphylococcus aureus
Genetic Insert: pEC1.2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167684 Copy
Species: Staphylococcus aureus
Genetic Insert: pEC1.2::asCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167683 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUBQ4-2::asCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167682 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167680 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUB4-2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: in addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167678 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74800 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74796 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Comments: Please note that pUC primers are not recommended for sequencing due to the inverted repeat of the Tnos sequence which forms a secondary structure and prevents sequencing reactions. If you need to sequence or PCR the Tnos region, digestion by AscI helps separates two Tnos sequences and prevents the secondary structure.
Proper citation: RRID:Addgene_74795 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74798 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74797 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74811 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74810 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74815 Copy
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