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Species: Caenorhabditis elegans
Genetic Insert: elt-2p::AID::nls::mcherry::2a::AID::PAR-6::VC155::tbb-2 3' in pLF3FShC
Vector Backbone Description: Backbone Marker:Michael Nonet, Addgene Plasmid #153083; Vector Backbone:pLF3FShC; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2025.02.28.640866v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_261136 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-C' linker-SL2-tTA(tet-OFF,DBD)-linker-QFAD-stop(TAA)-SEC(pDD364)-N' linker-rFRT3
Vector Backbone Description: Vector Backbone:pFH127; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255935 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-C' linker-SL2-LexA(DBD)-linker-QFAD-stop(TAA)-SEC(pDD364)-N' linker-rFRT3
Vector Backbone Description: Vector Backbone:pFH127; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255936 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-C' linker-GFP-SEC(pDD363)- N' linker-stop-rFRT3
Vector Backbone Description: Vector Backbone:pFH128; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255939 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-C' linker-SL2-QF2(DBD)-stop(TAA)-SEC(pDD364)-N' linker-rFRT3
Vector Backbone Description: Vector Backbone:pFH127; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255938 Copy
Species: Caenorhabditis elegans
Genetic Insert: SL2-ATG-rSapI-SpeI-SapI-AAG-SEC(pDD364 with Lox511I)-GGT
Vector Backbone Description: Vector Backbone:pFH127; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255980 Copy
Species: Caenorhabditis elegans
Genetic Insert: AAG-SEC(pDD382 with Lox2272)-GGT-rSapI-SpeI-SapI-ACG
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255982 Copy
Species: Caenorhabditis elegans
Genetic Insert: AAG-SEC(pDD363 with LoxP)-GGT-rSapI-SpeI-SapI-ACG
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255981 Copy
Species: Caenorhabditis elegans
Genetic Insert: AAG-SEC(pDD364 with Lox511I)-GGT-rSapI-SpeI-SapI-ACG
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255983 Copy
Species: Caenorhabditis elegans
Genetic Insert: AAG-SEC(pDD364 with Lox511I)-GGT-rSapI-SpeI-SapI-ACG-SL2
Vector Backbone Description: Vector Backbone:pFH174; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255986 Copy
Species: Caenorhabditis elegans
Genetic Insert: AAG-SEC(pDD382 with Lox2272)-GGT-rSapI-SpeI-SapI-ACG-SL2
Vector Backbone Description: Vector Backbone:pFH174; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255985 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SEC(pDD364)-AID-N' linker-SD-rFRT3
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255968 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SEC(pDD363)-ZF1-GFP-N' linker-SD-rFRT3
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255969 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SEC(pDD382)-mCherry-N' linker-SD-rFRT3
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: For unknown reasons, the DNA construct (pHW2159) seems toxic to the E. coli strain DH5α, often resulting in unexpected mutations. When transforming this plasmid into DH5α, we recommend selecting only small colonies and sequencing the plasmid following fresh miniprep from a bacterial culture to confirm absence of any mutations. To overcome this issue, the deposited bacterial stock of this plasmid is NEB® Turbo competent E. coli, which better tolerates this construct and more reliably yield plasmid DNA with the correct sequence.
Proper citation: RRID:Addgene_255961 Copy
Species: Caenorhabditis elegans
Genetic Insert: SL2-ATG-rSapI-SpeI-SapI-AAG-SEC(pDD363 with LoxP)-GGT
Vector Backbone Description: Vector Backbone:pFH127; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255978 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SEC(pDD364)-3X HA-N' linker-SD-rFRT3
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255970 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SEC(pDD364)-Dendra2-N' linker-SD-rFRT3
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255973 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SEC(pDD364)-KillerRed-N' linker-SD-rFRT3
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255974 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-C' linker-mCherry-SEC(pDD382)-N' linker-stop-rFRT3
Vector Backbone Description: Vector Backbone:pFH128; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255940 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-C' linker-mScarlet-I-C1-SEC(pDD382)-N' linker-stop-rFRT3
Vector Backbone Description: Vector Backbone:pFH128; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255942 Copy
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