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Species: Synthetic
Genetic Insert: RGR-GFP
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373158
Proper citation: RRID:Addgene_51057 Copy
Species: Synthetic
Genetic Insert: RGR-GFP
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373158
Proper citation: RRID:Addgene_51059 Copy
Species: Homo sapiens
Genetic Insert: ARAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24569458
Proper citation: RRID:Addgene_51078 Copy
Species: Halorubrum sp. TP009
Genetic Insert: eArchT3.0
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:6064; Vector Backbone:pAAV-CaMKIIa-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This is a cell filling variant for expression of eArchT3.0 and cytosolic EGFP.
Proper citation: RRID:Addgene_51110 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:custom; Backbone Size:5320; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled.
Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid.
Proper citation: RRID:Addgene_51083 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:custom; Backbone Size:5320; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled.
Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid.
Proper citation: RRID:Addgene_51082 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:Modified from Karl Deisseroth; Backbone Size:4590; Vector Backbone:pAAV-hSyn1-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled.
Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid.
Proper citation: RRID:Addgene_51085 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:custom; Backbone Size:5095; Vector Backbone:pAAV-CaMKIIa-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled.
Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid.
Proper citation: RRID:Addgene_51087 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:custom; Backbone Size:5095; Vector Backbone:pAAV-CaMKIIa-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled.
Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid.
Proper citation: RRID:Addgene_51086 Copy
Genetic Insert: LifeAct-mRFPruby
Vector Backbone Description: Backbone Marker:L. Naldini; Vector Backbone:pLenti.PGK:EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51009 Copy
Species: Synthetic
Genetic Insert: H2B-Dendra2
Vector Backbone Description: Vector Backbone:pLenti.CAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51005 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51126 Copy
Species: Homo sapiens
Genetic Insert: H2B-EGFP
Vector Backbone Description: Backbone Size:8344; Vector Backbone:pLenti.Syn(0.5).eGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51004 Copy
Genetic Insert: H2B-ChFP
Vector Backbone Description: Backbone Size:6686; Vector Backbone:pRRLsin.cPPT.PGKp.eGFP.wpre; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26209648
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51007 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8089; Vector Backbone:pSIR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Proper citation: RRID:Addgene_51128 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51127 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51254 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51253 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51256 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51250 Copy
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